The Relation of HPV Infection and Expression of p53 and p16 Proteins in Esophageal Squamous Cells Carcinoma

Author(s):Show less -

Aguiar Pastrez, Paula Roberta ; Mariano, Vania Sammartino ; da Costa, Allini Mafra ; Silva, Estela Maria ; Scapulatempo-Neto, Cristovam ; Guimaraes, Denise Peixoto ; Fava, Gilberto ; Zemi Neto, Said Abdala ; Nunes, Emily Montosa ; Sichero, Laura ; Villa, Luisa Lina ; Syrjanen, Kari Juhani ; Longatto-Filho, Adhemar

• Document type: Journal article
• Source: JOURNAL OF CANCER; v. 8, n. 6, p. 1062-1070, 2017.
• Web of Science Citations: 6

Abstract

GOAL: To investigate the HPV prevalence and characterize the expression of potential molecular surrogate markers of HPV infection in esophageal squamous cell carcinoma. MATERIALS AND METHODS: The prevalence of HPV in individuals with and without esophageal cancer (EC) was determined by using multiplex PCR; p16 and p53 protein levels were assessed by immunohistochemistry (IHC). RESULTS: High-risk HPV (hr-HPV) was found in the same frequency (13.8%) in esophageal squamous cell carcinoma (ESCC) and in healthy individuals. The p53 expression was positive in 67.5% of tumor tissue, 20.0% of adjacent non-tumoral tissue and 1.8% of normal esophageal tissue. p16 was positive in 11.6% of esophageal cancer cases and 4.7% of adjacent non-tumoral tissue. p16 was undetectable among control group samples. p53 and p16 levels were not significantly associated with the HPV status. CONCLUSIONS: These results suggest that hr-HPV types are not associated with the development of ESCC and that p53 and p16 protein expression have no relationship with HPV infection in normal or cancerous esophagus. (AU)

• FAPESP's process: 13/15968-0 - Association between high risk HPV infection and expression of proteins reported to esophageal carcionogenesis
• Grantee: Paula Roberta Aguiar Pastrez
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 08/57889-1 - Institute of Science and Technology to study Diseases Associated with Papillomavirus
• Grantee: Luisa Lina Villa
• Support Opportunities: Research Projects - Thematic Grants