Development of potent class II transactivator gene delivery systems capable of inducing de novo MHC II expression in human cells, in vitro and ex vivo

Author(s):Show less -

Palma, M. L. ; Duangkhae, P. ; Douradinha, B. ; Viana, I. F. T. ; Rigato, P. O. ; Dhalia, R. ; Mailliard, R. B. ; Barratt-Boyes, S. M. ; Nascimento, E. J. M. ; Oshiro, T. M. ; da Silva Duarte, A. J. ; Marques, E. T. A.

• Document type: Journal article
• Source: Gene Therapy; v. 24, n. 6, p. 342-352, JUN 2017.
• Web of Science Citations: 1

Abstract

Class II transactivator (CIITA) induces transcription of major histocompatibility complex (MHC) II genes and can potentially be used to improve genetic immunotherapies by converting non-immune cells into cells capable of presenting antigens to CD4(+) T cells. However, CIITA expression is tightly controlled and it remains unclear whether distinct non-immune cells differ in this transactivator regulation. Here we describe the development of gene delivery systems capable of promoting the efficient CIITA expression in non-immune cell lines and in primary human cells of an ex vivo skin explant model. Different human cell types undergoing CIITA overexpression presented high-level de novo expression of MHC II, validating the delivery systems as suitable tools for the CIITA evaluation as a molecular adjuvant for gene therapies. (AU)

• FAPESP's process: 12/22171-9 - In vitro cellular immune response of HIV-1 infected individuals induced by dendritic cells expressing the CIITA factor and HIV-1 gag protein fused to DC-LAMP
• Grantee: Mariana de Lucena Palma
• Support type: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 11/20649-6 - In vitro cellular immune response of HIV-1 infected individuals induced by dendritic cells expressing the CIITA factor and HIV-1 gag protein fused to DC-LAMP
• Grantee: Alberto José da Silva Duarte
• Support type: Regular Research Grants
• FAPESP's process: 11/12134-6 - IN VITRO CELLULAR IMMUNE RESPONSE OF HIV-1 INFECTED INDIVIDUALS INDUCED BY DENDRITIC CELLS EXPRESSING THE CIITA FACTOR AND HIV-1 GAG PROTEIN FUSED TO DC-LAMP
• Grantee: Mariana de Lucena Palma
• Support type: Scholarships in Brazil - Doctorate