Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparison of DNA Microarray, Loop-Mediated Isothermal Amplification (LAMP) and Real-Time PCR with DNA Sequencing for Identification of Fusarium spp. Obtained from Patients with Hematologic Malignancies

Full text
Author(s):
Show less -
de Souza, Marcela ; Matsuzawa, Tetsuhiro ; Sakai, Kanae ; Muraosa, Yasunori ; Lyra, Luzia ; Busso-Lopes, Ariane Fidelis ; Shafferman Levin, Anna Sara ; Schreiber, Angelica Zaninelli ; Mikami, Yuzuru ; Gonoi, Tohoru ; Kamei, Katsuhiko ; Moretti, Maria Luiza ; Trabasso, Plinio
Total Authors: 13
Document type: Journal article
Source: Mycopathologia; v. 182, n. 7-8, p. 625-632, AUG 2017.
Web of Science Citations: 2
Abstract

The performance of three molecular biology techniques, i.e., DNA microarray, loop-mediated isothermal amplification (LAMP), and real-time PCR were compared with DNA sequencing for properly identification of 20 isolates of Fusarium spp. obtained from blood stream as etiologic agent of invasive infections in patients with hematologic malignancies. DNA microarray, LAMP and real-time PCR identified 16 (80%) out of 20 samples as Fusarium solani species complex (FSSC) and four (20%) as Fusarium spp. The agreement among the techniques was 100%. LAMP exhibited 100% specificity, while DNA microarray, LAMP and real-time PCR showed 100% sensitivity. The three techniques had 100% agreement with DNA sequencing. Sixteen isolates were identified as FSSC by sequencing, being five Fusarium keratoplasticum, nine Fusarium petroliphilum and two Fusarium solani. On the other hand, sequencing identified four isolates as Fusarium non-solani species complex (FNSSC), being three isolates as Fusarium napiforme and one isolate as Fusarium oxysporum. Finally, LAMP proved to be faster and more accessible than DNA microarray and real-time PCR, since it does not require a thermocycler. Therefore, LAMP signalizes as emerging and promising methodology to be used in routine identification of Fusarium spp. among cases of invasive fungal infections. (AU)

FAPESP's process: 11/16205-5 - Identification of Fusarium species from clinical specimens from Campinas State University Hospital through the techniques of DNA microarray and loop-mediated isothermal amplification (LAMP).
Grantee:Marcela de Souza
Support Opportunities: Scholarships in Brazil - Master