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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Proteolytic processed form of CXCL12 abolishes migration and induces apoptosis in neural stem cells in vitro

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Adelita, Tais ; Stilhano, Roberta Sessa ; Han, SangWon ; Justo, Giselle Zenker ; Porcionatto, Marimelia
Total Authors: 5
Document type: Journal article
Source: STEM CELL RESEARCH; v. 22, p. 61-69, JUL 2017.
Web of Science Citations: 5

The subventricular zone (SVZ) of the adult mammalian brain hosts full potential neural stem cells (NSCs). NSCs are able to respond to extracellular signals in the brain, amplifying the pool of progenitor cells and giving rise to neuroblasts that showability to migrate towards an injury site. These signals can come fromvascular system, cerebrospinal fluid, glial cells, or projections of neurons in adjoining regions. CXCL12, a chemokine secreted after brain injury, reaches the SVZ in a gradient manner and drives neuroblasts towards the lesion area. Among many other molecules, matrix metalloproteinase 2 and 9 (MMP-2/9) are also released during brain injury. MMP-2/9 can cleave CXCL12 generating a new molecule, CXCL12(5-67), and its effects on NSCs viability is not well described. Here we produced recombinant CXCL12 and CXCL12(5-67) and evaluated their effect inmurine adultNSCsmigration and survival in vitro. We showed CXCL12(5-67) does not promote NSCsmigration, but does induce cell death. The NSC death induced by CXCL12(5-67) involves caspases 9 and 3/7 activation, implying the intrinsic apoptotic pathway in this phenomenon. Our evidences in vitromake CXCL12(5-67) and its receptor potential candidates for brain injuries and neurodegeneration studies. (C) 2017 The Author(s). Published by Elsevier B.V. (AU)

FAPESP's process: 12/00652-5 - Molecular mechanisms of neural stem cells migration, survival and differentiation
Grantee:Marimélia Aparecida Porcionatto
Support type: Regular Research Grants
FAPESP's process: 15/19231-8 - Wnt, SHH and Notch signalling crosstalk in the acquisition of stem cell phenotype by reactive astrocytes
Grantee:Marimélia Aparecida Porcionatto
Support type: Regular Research Grants
FAPESP's process: 11/11388-4 - CXCL12 chemokine effects on survival and differentiation of neural stem cell-derived neuroblasts
Grantee:Taís Adelita Morais de Almeida
Support type: Scholarships in Brazil - Doctorate