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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Diversity of aromatic hydroxylating dioxygenase genes in mangrove microbiome and their biogeographic patterns across global sites

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Author(s):
de Sousa, Sanderson T. P. ; Cabral, Lucelia ; Lacerda Junior, Gileno Vieira ; Oliveira, Valeria M.
Total Authors: 4
Document type: Journal article
Source: MICROBIOLOGYOPEN; v. 6, n. 4 AUG 2017.
Web of Science Citations: 3
Abstract

Aromatic hydrocarbons (AH), such as polycyclic aromatic hydrocarbons, are compounds largely found in nature. Aromatic-ring-hydroxylating dioxygenases (ARHD) are proteins involved in AH degradation pathways. We used ARHD functional genes from an oil-impacted mangrove area and compared their diversity with other sites around the world to understand the ARHD biogeographic distribution patterns. For this, a comprehensive database was established with 166 operational protein families (OPFs) from 1,758 gene sequences obtained from 15 different sites worldwide, of which twelve are already published studies and three are unpublished. Based on a deduced ARHD peptide sequences consensus phylogeny, we examined trends and divergences in the sequence phylogenetic clustering from the different sites. The taxonomic affiliation of the OPF revealed that Pseudomonas, Streptomyces, Variovorax, Bordetella and Rhodococcus were the five most abundant genera, considering all sites. The functional diversity analysis showed the enzymatic prevalence of benzene 1,2-dioxygenase, 3-phenylpropionate dioxygenase and naphthalene 1,2-dioxygenase, in addition to 10.98% of undefined category ARHDs. The ARHD gene correlation analysis among different sites was essentially important to gain insights on spatial distribution patterns, genetic congruence and ecological coherence of the bacterial groups found. This work revealed the genetic potential from the mangrove sediment for AH biodegradation and a considerable evolutionary proximity among the dioxygenase OPFs found in Antarctica and South America sites, in addition to high level of endemism in each continental region. (AU)

FAPESP's process: 13/22555-4 - Investigation of genes involved in biodegradation of aromatic hydrocarbons from metagenomic mangrove impacted with petroleum
Grantee:Sanderson Tarciso Pereira de Sousa
Support Opportunities: Scholarships in Brazil - Doctorate