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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Do chondroitin sulfates with different structures have different activities on chondrocytes and macrophages?

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Author(s):
da Cunha, Andre L. ; Aguiar, Jair A. K. ; Correa da Silva, Flavio S. ; Michelacci, Yara M.
Total Authors: 4
Document type: Journal article
Source: International Journal of Biological Macromolecules; v. 103, p. 1019-1031, OCT 2017.
Web of Science Citations: 8
Abstract

The aim of the present study was to investigate the activities of natural chondroitin sulfates (CS) with different structures on cultured chondrocytes and macrophages. CS were isolated from cartilages of bovine trachea (BT), porcine trachea (PT), chicken sternum (Ch) and skate (Sk). The preparations were 90-98% pure, with similar to 1% proteins, nucleic acids and keratan sulfate contaminants. Structural analysis of these CS and of commercial chondroitin 4- and 6-sulfate (C4S, C6S) have shown that most of their disaccharides are monosulfated, with varying proportions of 4- and 6-sulfation, and 2-7% non-sulfated disaccharides. Sk-CS and C6S contained detectable amounts of disulfated disaccharides. All the CS were polydisperse, with modal molecular weights of 26-135 kDa. These CS had anti-inflammatory activities on both chondrocytes and macrophages, but with different efficiencies. On horse and human chondrocytes, they reduced the IL-1 beta-induced liberation of NO and PGE(2), and on RAW 264.7 immortalized macrophage-like cell line, C4S, C6S, Ch and Sk-CS decreased the LPS-induced liberation of TNF-alpha, but did not affect IL-6. In contrast, on bone marrow derived macrophages, C4S, C6S, BT and PT-CS reduced the LPS-induced liberation of TNF-alpha, IL-6, IL-1 beta and NO, indicating that the RAW response to CS was different from that of primary macrophages. (C) 2017 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 10/16022-5 - Lysosomal enzymes, metalloproteinases and proteoglycans in Diabetes mellitus
Grantee:Yara Maria Corrêa da Silva Michelacci
Support Opportunities: Regular Research Grants
FAPESP's process: 13/07109-8 - Expression and activity of lysosomal enzymes in cultured cells exposed to conditions that mimic diabetes
Grantee:Yara Maria Corrêa da Silva Michelacci
Support Opportunities: Regular Research Grants