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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

NADPH OXIDASE CONTRIBUTES TO STREPTOZOTOCIN-INDUCED NEURODEGENERATION

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Author(s):
Ravelli, Katherine Garcia ; Rosario, Barbara dos Anjos ; Vasconcelos, Andrea Rodrigues ; Scavone, Cristoforo ; Camarini, Rosana ; Hernandes, Marina S. ; Britto, Luiz Roberto
Total Authors: 7
Document type: Journal article
Source: Neuroscience; v. 358, p. 227-237, SEP 1 2017.
Web of Science Citations: 3
Abstract

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the progressive loss of memory. The neurodegeneration induced by AD has been linked to oxidative damage. However, little is known about the involvement of NADPH oxidase 2 (Nox2), a multisubunit enzyme that catalyzes the reduction of oxygen to produce reactive oxygen species, in the pathogenesis of AD. The main purpose of this study was to investigate the involvement of Nox2 in memory, in AD-related brain abnormalities, oxidative damage, inflammation and neuronal death in the hippocampus in the streptozotocin (STZ)-induced AD-like state by comparing the effects of that drug on mice lacking gp91(phox-/-) and wild-type (Wt) mice. Nox2 gene expression was found increased in Wt mice after STZ injection. In object recognition test, Wt mice injected with STZ presented impairment in short- and long-term memory, which was not observed following Nox2 deletion. STZ treatment induced increased phosphorylation of Tau and increased amyloid-beta, apoptosis-inducing factor (AIF) and astrocyte and microglial markers expression in Wt mice but not in gp91(phox-/-). STZ treatment increased oxidative damage and pro-inflammatory cytokines' release in Wt mice, which was not observed in gp91(Phox-/-) mice. Nox2 deletion had a positive effect on the IL-10 baseline production, suggesting that this cytokine might contribute to the neuroprotection mechanism against STZ-induced neurodegeneration. In summary, our data suggest that the Nox2-dependent reactive oxygen species (ROS) generation contributes to the STZ-induced AD-like state. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 12/01444-7 - NADPH oxidase and Alzheimer's Disease
Grantee:Katherine Garcia Ravelli
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/16364-1 - NADPH oxidase in the model of Alzheimer's Disease by intracerebroventricular injection of streptozotocin
Grantee:Barbara dos Anjos Rosário
Support type: Scholarships in Brazil - Scientific Initiation