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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evidence of bone marrow downregulation in brain-dead rats

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Author(s):
Menegat, Laura ; Simas, Rafael ; Caliman, Julia M. ; Zanoni, Fernando L. ; Jacysyn, Jacqueline F. ; da Silva, Luiz Fernando F. ; Borelli, Primavera ; Moreira, Luiz Felipe P. ; Sannomiya, Paulina
Total Authors: 9
Document type: Journal article
Source: International Journal of Experimental Pathology; v. 98, n. 3, p. 158-165, JUN 2017.
Web of Science Citations: 1
Abstract

Experimental findings support the evidence of a persistent leucopenia triggered by brain death (BD). This study aimed to investigate leucocyte behaviour in bone marrow and blood after BD in rats. BD was induced using intracranial balloon catheter inflation. Sham-operated (SH) rats were trepanned only. Thereafter bone marrow cells were harvested every six hours from the femoral cavity and used for total and differential counts. They were analysed further by flow cytometry to characterize lymphocyte subsets, granulocyte adhesion molecules expression and apoptosis/necrosis {[} annexin V/propidium iodide (PI) protocol]. BD rats exhibited a reduction in bone marrow cells due to a reduction in lymphocytes (40%) and segmented cells (45%). Bone marrow lymphocyte subsets were similar in BD and SH rats (CD3, P = 0.1; CD4, P = 0.4; CD3/CD4, P = 0.4; CD5, P = 0.4, CD3/CD5, P = 0.2; CD8, P = 0.8). Expression of L-selectin and beta(2)-integrins on granulocytes did not differ (CD11a, P = 0.9; CD11b/c, P = 0.7; CD62L, P = 0.1). There were no differences in the percentage of apoptosis and necrosis (Annexin V, P = 0.73; PI, P = 0.21; Annexin V/PI, P = 0.29). In conclusion, data presented suggest that the downregulation of the bone marrow is triggered by brain death itself, and it is not related to changes in lymphocyte subsets, granulocyte adhesion molecules expression or apoptosis and necrosis. (AU)

FAPESP's process: 11/22803-2 - Study on the mobilization, activation, and apoptosis of bone marrow cells in a rat model of brain death
Grantee:Paulina Sannomiya
Support Opportunities: Regular Research Grants