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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

ESTRADIOL MODULATES LOCAL GUT INJURY INDUCED BY INTESTINAL ISCHEMIA-REPERFUSION IN MALE RATS

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Author(s):
Ricardo-da-Silva, Fernanda Yamamoto ; Fantozzi, Evelyn Thais ; Rodrigues-Garbin, Sara ; Oliveira-Filho, Ricardo Martins ; Vargaftig, Bernardo Boris ; Breithaupt-Faloppa, Ana Cristina ; de Lima, Wothan Tavares
Total Authors: 7
Document type: Journal article
Source: Shock; v. 48, n. 4, p. 477-483, OCT 2017.
Web of Science Citations: 7
Abstract

Intestinal ischemia and reperfusion (I/R) triggers a systemic inflammatory response characterized by leukocyte mobilization from the bone marrow, release of cytokines to the circulation, and increased microvascular permeability, leading to high mortality. Females have shown attenuated inflammatory response to trauma when compared with males, indicatinga role for female sex hormones in this process. Here, we have evaluated the effect of estradiol on the local gut injury induced by I/R in male rats. I/R was induced by the clamping of the superior mesenteric artery for 45 min, followed by 2 h of reperfusion. Agroup received 17 beta-estradiol (280 mu g/kg, i.v., single dose) at 30 min of ischemia. Morphometric analysis of the gut showed I/R induced a reduction of villous height that was prevented by estradiol. White blood cells, notably granulocytes, were mobilized from the circulation to the intestine by I/R, which was also prevented by estradiol treatment. Groups had the intestine wrapped in a plastic bag to collect intestinal fluid, where leukocytescount, TNF-alpha, and IL-10 levels were increased by I/R. Serum chemokines (CINC-1, MIP-1 alpha, MIP-2), ICAM-1 expression in the mesenteric tissue, and neutrophils spontaneous migration measured in vitro were also increased after I/R. Estradiol treatment reduced leukocytes numbers and TNF-alpha on intestinal fluid, serum chemokine release and also downregulated MIP-1 alpha, MIP-2 gene expression, and spontaneous in vitro neutrophil migration. In conclusion, estradiol blunts intestinal injury induced by I/R by modulating chemokines release and leukocyte trafficking. (AU)

FAPESP's process: 13/15291-0 - Effect of intestinal ischemia and reperfusion on intestinal contraction, generation of inflammatory mediators and mesenteric microcirculation. role of gender and lymphatic system
Grantee:Wothan Tavares de Lima
Support Opportunities: Regular Research Grants