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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation and chemical characterization of agelaiatoxin8 (AvTx8) from Agelaia vicina wasp venom and its biological effects on GABA neurotransmission

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Author(s):
Pizzo, Andrea B. [1] ; Beleboni, Rene O. [2] ; Gomes Carolino, Ruither O. [3] ; de Oliveira, Luciana [1] ; Miranda, Antonio [4] ; Coutinho-Netto, Joaquim [3] ; Fontana, Andreia C. K. [5] ; dos Santos, Wagner Ferreira [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Coll Philosophy Sci & Literature FFCLRP, Lab Neurobiol & Venoms, FFCLRPAv, Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Ribeirao Preto UNAERP, Biotecnol Dept, Ribeirao Preto - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Biochem & Immunol, Lab Neurochem, Ribeirao Preto - Brazil
[4] Univ Fed Sao Paulo, Dept Biophys, Sao Paulo - Brazil
[5] Drexel Univ, Dept Physiol & Pharmacol, Coll Med, Philadelphia, PA 19104 - USA
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY; v. 31, n. 10 OCT 2017.
Web of Science Citations: 0
Abstract

Arthropod venoms are sources of molecules that may be useful tools to investigate molecular mechanisms of putative new medicines and laboratory drugs. Here we show the effects of the compound agelaiatoxin-8 (AVTx8), isolated from Agelaia vicina venom, on -aminobutyric acid (GABA) neurotransmission in rat brain synaptosomes. Analysis reveals that AvTx8 is composed by 14 amino acid residues with a molecular weight (MW) of 1567Da. AvTx8 increased GABA release and inhibited GABA uptake in synaptosomes from rat cerebral cortex. AvTx8 inhibited GABA uptake and increased GABA release in the presence of Ca+, Na+, and K+ channel blockers, suggesting that it acts directly on GABA transporters. In addition, AvTx8 significantly decreases GABA binding in synaptic membranes from rat brain cortex, suggesting that it also modulates the activity of GABA receptors. Moreover, AvTx8 decreased GAT-1- and GAT-3-mediated GABA uptake in transfected COS-7 cells. Accordingly, we suggest that AvTx8 modulates GABA neurotransmission and might provide a novel entry point for identifying a new class of GABA-modulating neuroprotective drugs. (AU)