RNA expression analysis in dental pulp cells cultured by cell sheets
| Full text | |
| Author(s): |
Penna, Vanessa
[1, 2]
;
Lipay, Monica V. N.
[3, 4, 5]
;
Duailibi, Monica T.
[1, 3, 6]
;
Duailibi, Silvio E.
[1, 3]
Total Authors: 4
|
| Affiliation: | [1] Ctr Cellular & Mol Therapy, CTCMol, Sao Paulo, SP - Brazil
[2] Fed Univ Sao Paulo EPM UNIFESP, Paulista Sch Med, Grad Translat Surg Program, Sao Paulo, SP - Brazil
[3] EPM UNIFESP, Surg Dept, Translat Surg Div, Sao Paulo, SP - Brazil
[4] Jundiai Fac Med, Dept Morphol & Basic Pathol, Sao Paulo, SP - Brazil
[5] EPM UNIFESP, Med Dept, Endocrinol Div, Sao Paulo, SP - Brazil
[6] Univ Santo Amaro UNISA, Dept Implantol, Sao Paulo, SP - Brazil
Total Affiliations: 6
|
| Document type: | Review article |
| Source: | FUTURE SCIENCE OA; v. 1, n. 3 NOV 2015. |
| Web of Science Citations: | 2 |
| Abstract | |
Tissue engineering aims at developing the necessary technological strategies for replacement or regeneration tissues. However, the number of cells required is much greater than the number obtained from a cell source. Expanding the cells' number in cell culture for a long period is required until the necessary amount of cells is obtained. While in culture, cells often go unwanted differentiation. Little attention has been given to the use of proteolytic enzymes in cell passage. Review the importance of extracellular matrix and surface proteins for cell behavior and the possible mechanisms of cellular changes that may occur due to the use of proteolytic enzymes is an essential issue. Possible alternative to replace these enzymes in cell passage has also been developed. Tissue engineering aims at developing strategies for tissue replacement. However, the number of cells required is much greater than the number obtained from a cell source. Expanding the cells' number in cell culture for a long period is required until the necessary amount of cells is obtained but, cells often go unwanted differentiation. Proteolytic enzymes are commonly used in culture and it might lead to such cellular changes. This article aims at reviewing the possible mechanisms of cellular changes that may occur due to the use of proteolytic enzymes and possible alternatives to replace the use of those enzymes. (AU) | |
| FAPESP's process: | 13/06877-1 - Glucose and glutamine in cell proliferation and viability |
| Grantee: | Monica Talarico Duailibi |
| Support type: | Regular Research Grants |
| FAPESP's process: | 13/00288-4 - RNA expression analysis in dental pulp cells cultured by cell sheets |
| Grantee: | Silvio Eduardo Duailibi |
| Support type: | Regular Research Grants |