Stem cells from human-exfoliated deciduous teeth reduce tissue-infiltrating inflammatory cells improving clinical signs in experimental autoimmune encephalomyelitis

Author(s)

Rossato, Cristiano ^[1] ; Brandao, Wesley N. ^[1] ; Castro, Sandra B. R. ^[2] ; de Almeida, Danilo C. ^[1] ; Maranduba, Carlos M. C. ^[2] ; Camara, Niels O. S. ^[1] ; Peron, Jean P. S. ; Silva, Fernando S. ^[2]

Affiliation

^[1] Univ Sao Paulo, Sao Paulo - Brazil

^[2] Univ Fed Juiz de Fora, BR-36036900 Juiz De Fora, MG - Brazil

• Document type: Journal article
• Source: BIOLOGICALS; v. 49, p. 62-68, SEP 2017.
• Web of Science Citations: 6

Abstract

Stem cells from human exfoliated deciduous teeth (SHED) have great therapeutic potential and here, by the first time, we evaluated their immunomodulatory effect on experimental model of autoimmune encephalomyelitis (EAE). Specifically, we investigated the effect of SHED administration on clinical signs and cellular patterns in EAE model using Foxp3 GFP + transgenic mice (C57BI/6-Foxp3GFP). The results showed that SHED infusion ameliorated EAE clinical score with reduced number of infiltrating IFN-gamma(+)CD8(+), IL-4(+)CD8(+), IFN-gamma(+)CD4(+) and IL-4(+)CD4(+) T cells into the central nervous system (CNS). In addition, we observed that SHED promoted a significant increase in CD4(+)FOXP3+ T cells population in the spleen of EAE-affected animals. Taken together, our results provide strong evidence that SHED can modulate peripherally the CD4(+) T cell responses suggesting that SHED would be explored as part of cellular therapy in autoimmune diseases associated with CNS. (C) 2017 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 11/18703-2 - The role of Indoleamine-2,3-dioxigenase and the Triptophan - Kynurenines axis through NMDA receptors over the immune response in the EAE and stroke model
• Grantee: Jean Pierre Schatzmann Peron
• Support Opportunities: Research Grants - Young Investigators Grants