Slc11a1 (Nramp-1) gene modulates immune-inflammation genes in macrophages during pristane-induced arthritis in mice

Author(s):Show less -

Correa, Mara A. ^[1] ; Canhamero, Tatiane ^[1] ; Borrego, Andrea ^[1] ; Katz, Iana S. S. ^[2] ; Jensen, Jose R. ^[1] ; Guerra, Jose Luiz ^[3] ; Cabrera, Wafa H. K. ^[1] ; Starobinas, Nancy ^[1] ; Fernandes, Jussara G. ^[1] ; Ribeiro, Orlando G. ^[1] ; Ibanez, Olga M. ^[1] ; De Franco, Marcelo ^{[2, 1]}

Affiliation

^[1] Inst Butantan, Lab Imunogenet, Ave Vital Brasil 1500, BR-05503000 Sao Paulo, SP - Brazil

^[2] Inst Pasteur, Sao Paulo - Brazil

^[3] Univ Anhembi Morumbi, Sao Paulo - Brazil

• Document type: Journal article
• Source: Inflammation Research; v. 66, n. 11, p. 969-980, NOV 2017.
• Web of Science Citations: 4

Abstract

Pristane-induced arthritis (PIA) in AIRmax mice homozygous for Slc11a1 R and S alleles was used to characterize the influence of Slc11a1 gene polymorphism on immune responses during disease manifestation. Previous reports demonstrated that the presence of the Slc11a1 S allele increased the incidence and severity of PIA in AIRmax (SS) , suggesting that this gene could interact with inflammatory loci to modulate PIA. We investigated the effects of Slc11a1 alleles on the activation of phagocytes during PIA. Mice were injected intraperitoneally with two doses of 0.5 mL of mineral oil pristane at 60-day intervals. Arthritis development was accompanied for 180 days. AIRmax (SS) mice showed differential peritoneal macrophage gene expression profiles during PIA, with higher expression and production of H2O2, NO, IL-1 beta, IL-6, TNF-alpha, and several chemokines. The presence of the Slc11a1 R allele, on the other hand, diminished the intensity of macrophage activation, restricting arthritis development. Our data demonstrated the fine-tuning roles of Slc11a1 alleles modulating macrophage activation, and consequent PIA susceptibility, in those mouse lines. (AU)

• FAPESP's process: 09/18414-0 - THE GENE Slc11a1 PARTICIPATION IN THE MODULATION OF THE IMMUNE RESPONSE ON PRISTANE-INDUCED ARTHRITIS IN GENETICALLY SELECTED MICE FOR THE ACUTE INFLAMMATORY RESPONSE
• Grantee: Mara Adriana Corrêa Giacoia
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/18060-2 - Research of genes regulating the severity of acute inflammation and their interactions with micro RNAs in the development of experimental arthritis
• Grantee: Marcelo de Franco
• Support Opportunities: Regular Research Grants