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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Physical training improves visceral adipose tissue health by remodelling extracellular matrix in rats with estrogen absence: a gene expression analysis

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Author(s):
Duarte, Fernanda O. [1, 2] ; Gomes-Gatto, Camila do Valle [2] ; Oishi, Jorge C. [1] ; Lino, Anderson Diogo de S. [1] ; Stotzer, Uliana S. [2] ; Rodrigues, Maria Fernanda C. [2] ; Gatti da Silva, Guilherme H. [2] ; Selistre-de-Araujo, Heloisa S. [2]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Carlos UFSCar, Lab Nutr & Metab Appl Exercise, Dept Phys Educ & Human Motr, Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos UFSCar, Dept Physiol Sci, Lab Biochem & Mol Biol, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: International Journal of Experimental Pathology; v. 98, n. 4, p. 203-213, AUG 2017.
Web of Science Citations: 4
Abstract

Adipose tissue development is associated with modifications involving extracellular matrix remodelling, and metalloproteinases play a significant role in this process. Reduced circulating sexual hormones cause impacts on the size, morphology and functions of the adipose tissue, increasing susceptibility to diseases. This study investigated whether exercise training may be an alternative strategy to combat the effects promoted by estrogen decay through modulation in gene expression patterns in the extracellular matrix (ECM) of visceral adipose tissue of ovariectomized rats. Nulliparous rats (n=40) were randomly distributed into four groups (n=10/group): sham sedentary (Sh-S), sham resistance training (Sh-Rt), ovariectomized sedentary (Ovx-S) and ovariectomized resistance training (Ovx-Rt). The Sh-S animals did not have any type of training. The body mass and food intake, ECM gene expression, gelatinase MMP-2 activity and adipocyte area were measured. A lack of estrogen promoted an increase in body mass, food intake and the visceral, parametrial and subcutaneous adipocyte areas. The ovariectomy upregulated the expression of MMP-2, MMP-9, TGF-, CTGF, VEGF-A and MMP-2 activity. On the other hand, resistance training decreased the body mass, food intake and the adipocyte area of the three fat depots analysed; upregulated TIMP-1, VEGF-A and MMP-2 gene expression; downregulated MMP-9, TGF- and CTGF gene expression; and decreased the MMP-2 activity. We speculate that resistance training on a vertical ladder could play an important role in maintaining and remodelling ECM by modulation in the ECM gene expression and MMP-2 activity, avoiding its destabilization which is impaired by the lack of estrogen. (AU)