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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sex steroids modulate morphological and functional features of the bovine oviduct

Full text
Author(s):
Gonella-Diaza, Angela Maria [1] ; Mesquita, Fernando Silveira [2] ; da Silva, Kaue Ribeiro [1] ; de Carvalho Balieiro, Julio Cesar [1] ; dos Santos, Nilton Pedro [3] ; Pugliesi, Guilherme [3] ; Strefezzi, Ricardo de Francisco [3] ; Binelli, Mario [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Sao Paulo - Brazil
[2] Univ Fed Pampa, Curso Med Vet, Uruguaiana, RS - Brazil
[3] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Cell and Tissue Research; v. 370, n. 2, p. 319-333, NOV 2017.
Web of Science Citations: 7
Abstract

In cattle, the oviduct plays a major role in the reproductive process; however, molecular control of oviduct receptivity to the embryo is poorly understood. A model for receptivity based on size of the pre-ovulatory follicle (POF) was used to compare oviductal morphology, cellular proliferation, and candidate transcript abundance. Growth of the POF of Nelore (Bos indicus) cows was manipulated to produce two groups: a large POF-large corpus luteum (CL) group (LF-LCL; greater receptivity) and a small POF-small CL group (SF-SCL). Samples of the ampulla and isthmus ipsilateral and contralateral to CL were collected 4 days after GnRH-induced ovulation. Tissues were either embedded in paraffin for Harris-Hematoxylin and Eosin and periodic acid-Schiff staining and KI67 immunostaining, followed by morphological analyses, or stored at -80 degrees C for RNA extraction, cDNA synthesis, and qPCR analyses. The effects of group (LF-LCL and SF-SCL), region (ampulla and isthmus), and side (ipsilateral and contralateral) were analyzed using three-way nested ANOVA. The ipsilateral ampulla of the LF-LCL group presented more primary mucosal folds, a greater mucosal-folding grade and luminal perimeter, and more secretory cells and proliferating cells when compared with the ampulla of the SF-SCL group and with the contralateral ampulla of both groups. There were no morphological differences in the isthmus between groups and sides. Changes in transcript abundance are suggestive of LF-LCL-stimulated secretory activity. In summary, ovulation of a larger POF generates a periovulatory endocrine milieu that modulates morphological and functional features of the bovine oviduct which may support embryo survival and development. (AU)

FAPESP's process: 11/03226-4 - Signatures of receptivity
Grantee:Mario Binelli
Support Opportunities: Research Projects - Thematic Grants