HPV infection and p53 and p16 expression in esophageal cancer: are they prognostic factors?

Author(s):Show less -

da Costa, Allini Mafra ^{[1, 2]} ; Guerreiro Fregnani, Jose Humberto Tavares ^[1] ; Aguiar Pastrez, Paula Roberta ^{[1, 3]} ; Mariano, Vania Sammartino ^{[1, 3]} ; Silva, Estela Maria ^{[1, 3]} ; Neto, Cristovam Scapulatempo ^[1] ; Guimaraes, Denise Peixoto ^{[3, 4]} ; Villa, Luisa Lina ^{[5, 6]} ; Sichero, Laura ^[5] ; Syrjanen, Kari Juhani ^{[1, 3, 7]} ; Longatto-Filho, Adhemar ^{[1, 3, 8, 9, 10]}

Affiliation

^[1] Pius XII Fdn, Teaching & Res Inst, Barretos Canc Hosp, Rua Antenor Duarte Vilela 1331, BR-14784400 Sao Paulo - Brazil

^[2] Pius XII Fdn, Canc Registry, Barretos Canc Hosp, Sao Paulo - Brazil

^[3] Pius XII Fdn, Barretos Canc Hosp, Mol Oncol Res Ctr, Sao Paulo - Brazil

^[4] Pious XII Fdn, Dept Endoscopy, Barretos Canc Hosp, Sao Paulo - Brazil

^[5] ICESP, Mol Biol Lab, Ctr Translat Res Oncol, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Dept Radiol & Oncol, Sch Med, Sao Paulo - Brazil

^[7] Biohit Oyj, Dept Clin Res, Helsinki - Finland

^[8] Univ Sao Paulo, Med Lab Med Invest LIM 14, Dept Pathol, Fac Med, Sao Paulo - Brazil

^[9] Univ Minho, Res Inst Life & Hlth Sci ICVS, Braga - Portugal

^[10] Govt Portugal, ICVS Associated Lab 3Bs, Braga - Portugal

• Document type: Journal article
• Source: INFECTIOUS AGENTS AND CANCER; v. 12, OCT 13 2017.
• Web of Science Citations: 3

Abstract

Background: Esophageal squamous cell carcinoma (ESCC) is a highly lethal malignant tumor. Currently, Human papillomavirus (HPV) is suggested as a potential risk factor for esophageal cancer (EC) in addition to the classic risk factors, alcohol and tobacco, but this hypothesis still remains contradictory. We sought to investigate wether HPV and well-known biomarkers (p16 and p53) and patient-related factors that may have impact on survival of ESCC. Methods: We conducted a prospective cohort study. By using multiplex PCR, we determined the prevalence of high risk HPV in ESCC, and evaluated the immunohistochemical expression of p16 and p53, molecular markers related to esophageal carcinogenesis in order to verify the potential influence of these variables in patients's survival. Survival rates were estimated using Kaplan-Meier methods. A multivariate confirmatory model was performed using Cox proportional hazards regression. Results: Twelve (13.8%) of 87 patients were HPV-DNA positive. Positive reactions of p16 and p53 were 10.7% and 68.6%, respectively. Kaplan-Meier analysis indicated that men (p = 0.025) had poor specific-cancer survival and a shorter progression-free survival (p = 0.050) as compared to women; III or IV clinical stage (p < 0.019) had poor specific-cancer survival and a shorter progression-free survival (p < 0.001) compared to I and II clinical stage; not submitted to surgery (< 0.001) and not submitted to chemoradiotherapy (p = 0.039) had a poor specific-cancer survival, as well. The multivariate analysis showed that HPV, p16 and p53 status are not predictive parameters of progression-free and specific-cancer survival. Conclusion: HPV infection and p53 and p16 expression are not prognostic factors in ESCC. (AU)

• FAPESP's process: 08/57889-1 - Institute of Science and Technology to study Diseases Associated with Papillomavirus
• Grantee: Luisa Lina Villa
• Support Opportunities: Research Projects - Thematic Grants