Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Low Concentration of 5-Fluorouracil Increases the Effectiveness of Tumor RNA to Activate Murine Dendritic Cells

Full text
de Camargo, Marcela Rodrigues [1] ; Gorgulho, Carolina Mendonca [1] ; Rodrigues, Cecilia Pessoa [2] ; Penitenti, Marcimara [3] ; Longo Frederico, Juliana Cristina [1] ; Marchesan Rodrigues, Maria Aparecida [1] ; Kaneno, Ramon [2]
Total Authors: 7
[1] Sao Paulo State Univ UNESP, Sch Med Botucatu, Dept Pathol, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Microbiol & Immunol, Botucatu, SP - Brazil
[3] Hosp Amaral Carvalho, Lab Flow Cytometry Jau, Jau - Brazil
Total Affiliations: 3
Document type: Journal article
Source: CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS; v. 32, n. 8, p. 302-308, OCT 2017.
Web of Science Citations: 0

Aim: Considering the central role of dendritic cells (DCs) on the development of an antitumor immune response, in this study we used a murine model to evaluate how DC transfection with drug-treated tumor cell RNA changes their phenotype, and whether transfection enhances the in vivo effectiveness of a DC-based antitumor vaccine. Materials and Methods: MC-38 colorectal tumor cells were pretreated with the minimum effective concentration of 5-fluorouracil (5-FU), then their total RNA was extracted and transfected into DCs. These DCs were inoculated into C57Bl/6 mice bearing subcutaneous MC-38 tumor. Results: DC transfection with drug-treated tumor RNA increases the percentages of CD40(+) (from 37.6% to 61.4%), CD86(+) (from 39.8% to 53.4%), and major histocompatibility complex class II+ (from 51.2% to 75.3%) cells, whereas significantly increases the in vivo generation of interferon-c producer lymphocytes. Conclusion: These results reinforce our view that treatment of tumor cells with 5-FU induces transcriptional changes that can be transferred to DCs by RNA transfection, enhancing their ability to stimulate an antitumor response. (AU)

FAPESP's process: 09/18331-8 - Chemoimmunomodulation with antineoplastic agents and generation of dendritic cell vaccine
Grantee:Ramon Kaneno
Support Opportunities: Regular Research Grants
FAPESP's process: 10/06013-9 - In vivo role of dendritic cell vaccines sensitized with mRNA from MC-38 cells pre-treated with antineoplastic agents at minimum effective doses
Grantee:Marcela Rodrigues de Camargo
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 11/05258-0 - Immunomodulatory effect of low concentrations of antineoplastic
Grantee:Juliana Cristina Longo Frederico
Support Opportunities: Scholarships in Brazil - Master