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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Simultaneous analysis of multiple T helper subsets in leprosy reveals distinct patterns of Th1, Th2, Th17 and Tregs markers expression in clinical forms and reactional events

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Author(s):
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Soriani Azevedo, Michelle de Campos [1] ; Marques, Heloisa [2] ; Binelli, Larissa Sarri [2] ; Vieira Malange, Mariana Silva [2] ; Devides, Amanda Carreira [2] ; Silva, Eliane Aparecida [1] ; Vincenzi Fachin, Luciana Raquel [1] ; Ghidella, Cassio Cesar [3] ; Soares, Cleverson Teixeira [1] ; Garlet, Gustavo Pompermaier [4] ; Rosa, Patricia Sammarco [1] ; Fernandes Belone, Andrea de Farias [1] ; Favaro Trombone, Ana Paula [2]
Total Authors: 13
Affiliation:
[1] Inst Lauro de Souza Lima, Rodovia Comandante Joao Ribeiro de Barros, BR-17034971 Bauru, SP - Brazil
[2] Univ Sagrado Coracao, Ctr Ciencias Saude, Rua Irma Arminda 10-50, BR-17011160 Bauru, SP - Brazil
[3] Ctr Referencia Hanseniase & TB, Rua Pedro Guimaraes S-N, BR-78710760 Rondonopolis, Mato Grosso - Brazil
[4] Univ Sao Paulo, Dept Ciencias Biol, Fac Odontol Bauru, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: MEDICAL MICROBIOLOGY AND IMMUNOLOGY; v. 206, n. 6, p. 429-439, DEC 2017.
Web of Science Citations: 2
Abstract

Leprosy is a chronic infectious disease caused by Mycobacterium leprae. Previous studies have demonstrated that the difference among clinical forms of leprosy can be associated with the immune response of patients, mainly by T helper (Th) and regulatory T cells (Tregs). Then, aiming at clarifying the immune response, the expression of cytokines related to Th1, Th2, Th17 and Tregs profiles were evaluated by qPCR in 87 skin biopsies from leprosy patients. Additionally, cytokines and anti-PGL-1 antibodies were determined in serum by ELISA. The results showed that the expression of various targets (mRNA) related to Th1, Th2, Th17 and Tregs were significantly modulated in leprosy when compared with healthy individuals, suggesting the presence of a mixed profile. In addition, the targets related to Th1 predominated in the tuberculoid pole and side and Th2 and Tregs predominated in the lepromatous pole and side; however, Th17 targets showed a mixed profile. Concerning reactional events, Tregs markers were decreased and IL-15 was increased in reversal reaction and IL-17F, CCL20 and IL-8 in erythema nodosum leprosum, when compared with the respective non-reactional leprosy patients. Additionally, ELISA analysis demonstrated that IL-22, IL-6, IL-10 and anti-PGL-1 antibody levels were significantly higher in the serum of patients when compared with healthy individuals, and IL-10 and anti-PGL-1 antibodies were also increased in the lepromatous pole and side. Together, these results indicate that Th1, Th2 and Th17 are involved in the determination of clinical forms of leprosy and suggest that decreased Tregs activity may be involved in the pathogenesis of reactional events. (AU)

FAPESP's process: 09/06122-5 - Evaluation of the regulatory T and Th17 cells role in the human and experimental leprosy: correlation with leprosy spectrum, metalloproteinases and their inhibitors expression, and interaction with Th1/Th2 paradigm
Grantee:Ana Paula Favaro Trombone Garlet
Support type: Research Grants - Young Investigators Grants