| Full text | |
| Author(s): Show less - |
Schaefer, Miriam
[1]
;
Granato, Daniela C.
[2]
;
Krossa, Sebastian
[3]
;
Bartels, Anne-Kathrin
[1]
;
Yokoo, Sami
[2]
;
Dusterhoft, Stefan
[4]
;
Koudelka, Tomas
[5]
;
Scheidig, Axel J.
[3]
;
Tholey, Andreas
[5]
;
Paes Leme, Adriana F.
[2]
;
Groetzinger, Joachim
[1]
;
Lorenzen, Inken
[1, 3]
Total Authors: 12
|
| Affiliation: | [1] Univ Kiel, Inst Biochem, Olshausenstr 40, D-24118 Kiel - Germany
[2] CNPEM, LNBio, Lab Nacl Biociencias, Lab Espectrometria Massas, BR-13083970 Campinas, SP - Brazil
[3] Inst Zool, Dept Biol Struct, Kiel - Germany
[4] Sir William Dunn Sch Pathol, Oxford - England
[5] Univ Kiel, Inst Expt Med, Div Systemat Proteome Res, Kiel - Germany
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | FEBS Letters; v. 591, n. 21, p. 3567-3587, NOV 2017. |
| Web of Science Citations: | 4 |
| Abstract | |
The shedding of ectodomains is a crucial mechanism in many physiological and pathological events. A disintegrin and metalloprotease-17 (ADAM17) is a key sheddase involved in essential processes, such as development, regeneration, and immune defense. ADAM17 exists in two conformations which differ in their disulfide connection in the membrane-proximal domain (MPD). Protein-disulfide isomerases (PDIs) on the cell surface convert the open MPD into a rigid closed form, which corresponds to inactive ADAM17. ADAM17 is expressed in its open activatable form in the endoplasmic reticulum (ER) and consequently must be protected against ER-resident PDI activity. Here, we show that the chaperone 78-kDa glucose-regulated protein (GRP78) protects the MPD against PDI-dependent disulfide-bond isomerization by binding to this domain and, thereby, preventing ADAM17 inhibition. (AU) | |
| FAPESP's process: | 11/02267-9 - Identifying ADAM17 partners and mapping the domains responsible for its interaction with other proteins. |
| Grantee: | Daniela Campos Granato |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 09/54067-3 - Acquisition of a mass spectrometer coupled to a liquid chromatography system for increasing the capacity to meet the needs of users and for making new technologies available in the Laboratory of Mass Spectrometry |
| Grantee: | Adriana Franco Paes Leme |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 10/19278-0 - Study of regulation of ADAMs in oral cancer |
| Grantee: | Adriana Franco Paes Leme |
| Support Opportunities: | Research Grants - Young Investigators Grants |