| Full text | |
| Author(s): |
dos Reis, Mariana Bisarro
[1, 2, 3]
;
Barros-Filho, Mateus Camargo
[1, 2]
;
Marchi, Fabio Albuquerque
[1, 2]
;
Beltrami, Caroline Moraes
[1, 2]
;
Kuasne, Hellen
[1, 2]
;
Lopes Pinto, Clovis Antonio
[4]
;
Ambatipudi, Srikant
[5, 6]
;
Herceg, Zdenko
[5]
;
Kowalski, Luiz Paulo
[7]
;
Rogatto, Silvia Regina
[3, 8, 9]
Total Authors: 10
|
| Affiliation: | [1] AC Camargo Canc Ctr, CIPE, Int Res Ctr, BR-01509010 Sao Paulo, SP - Brazil
[2] Natl Inst Sci & Technol Oncogen, BR-01509010 Sao Paulo, SP - Brazil
[3] Sao Paulo State Univ, Dept Urol, Fac Med, UNESP, BR-18618970 Botucatu, SP - Brazil
[4] AC Camargo Canc Ctr, Dept Pathol, BR-01509010 Sao Paulo, SP - Brazil
[5] IARC, Epigenet Grp, F-69372 Lyon - France
[6] Univ Bristol, Integrat Epidemiol Unit, MRC, Bristol BS8 1TH, Avon - England
[7] AC Camargo Canc Ctr, Dept Head & Neck Surg & Otorhinolaryngol, BR-01509010 Sao Paulo, SP - Brazil
[8] Univ Southern Denmark, Dept Clin Genet, Vejle Hosp, DK-7100 Vejle - Denmark
[9] Univ Southern Denmark, Inst Reg Hlth Res, Vejle Hosp, DK-7100 Vejle - Denmark
Total Affiliations: 9
|
| Document type: | Journal article |
| Source: | JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM; v. 102, n. 11, p. 4089-4099, NOV 1 2017. |
| Web of Science Citations: | 12 |
| Abstract | |
Context: Even though the majority of well-differentiated thyroid carcinoma (WDTC) is indolent, a number of cases display an aggressive behavior. Cumulative evidence suggests that the deregulation of DNA methylation has the potential to point out molecular markers associated with worse prognosis. Objective: To identify a prognostic epigenetic signature in thyroid cancer. Design: Genome-wide DNA methylation assays (450k platform, Illumina) were performed in a cohort of 50 nonneoplastic thyroid tissues (NTs), 17 benign thyroid lesions (BTLs), and 74 thyroid carcinomas (60 papillary, 8 follicular, 2 Hurthle cell, 1 poorly differentiated, and 3 anaplastic). A prognostic classifier for WDTC was developed via diagonal linear discriminant analysis. The results were compared with The Cancer Genome Atlas (TCGA) database. Results: A specific epigenetic profile was detected according to each histological subtype. BTLs and follicular carcinomas showed a greater number of methylated CpG in comparison with NTs, whereas hypomethylation was predominant in papillary and undifferentiated carcinomas. A prognostic classifier based on 21 DNA methylation probes was able to predict poor outcome in patients with WDTC (sensitivity 63%, specificity 92% for internal data; sensitivity 64%, specificity 88% for TCGA data). High-risk score based on the classifier was considered an independent factor of poor outcome (Cox regression, P < 0.001). Conclusions: The methylation profile of thyroid lesions exhibited a specific signature according to the histological subtype. A meaningful algorithm composed of 21 probes was capable of predicting the recurrence in WDTC. (AU) | |
| FAPESP's process: | 08/57887-9 - National Institute of Oncogenomics |
| Grantee: | Luiz Paulo Kowalski |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/20748-5 - Diagnostic and prognostic markers useful in the clinical practice for patients with thyroid nodules: validation of previous findings and functional studies |
| Grantee: | Silvia Regina Rogatto |
| Support Opportunities: | Regular Research Grants |