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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Triiodothyronine modulates the expression of leptin and adiponectin in 3T3-L1 adipocytes

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Author(s):
de Oliveira, Miriane [1] ; De Sibio, Maria Teresa [1] ; Castro Olimpio, Regiane Marques [1] ; Fontes Moretto, Fernanda Cristina [1] ; Melo Luvizotto, Renata de Azevedo [2] ; Nogueira, Celia Regina [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista, Botucatu, SP - Brazil
[2] Univ Fed Mato Grosso, Cuiaba, MT - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Einstein (São Paulo); v. 13, n. 1, p. 72-78, JAN-MAR 2015.
Web of Science Citations: 6
Abstract

Objective To study the effect of different doses of triiodothyronine on gene expression of the adipokines leptin and adiponectin, at different times, and to evaluate the difference in expression between the two adipokines in each group. Methods 3T3-L1 adipocytes were incubated with triiodothyronine at physiological dose (10nM) and supraphysiological doses (100nM or 1,000nM), or without triiodothyronine (control, C) for 0.5, 6, or 24 hours. Leptin and adiponectin mRNA was detected using real-time polymerase chain reaction (RT-PCR). One-way analyses of variance, Tukey’s test or Student’s t test, were used to analyze data, and significance level was set at 5%. Results Leptin levels decreased in the 1,000nM-dose group after 0.5 hour. Adiponectin levels dropped in the 10nM-dose group, but increased at the 100nM dose. After 6 hours, both genes were suppressed in all hormone concentrations. After 24 hours, leptin levels increased at 10, 100 and 1,000nM groups as compared to the control group; and adiponectin levels increased only in the 100nM group as compared to the control group. Conclusion These results demonstrated fast actions of triiodothyronine on the leptin and adiponectin expression, starting at 0.5 hour, at a dose of 1,000nM for leptin and 100nM for adiponectin. Triiodothyronine stimulated or inhibited the expression of adipokines in adipocytes at different times and doses which may be useful to assist in the treatment of obesity, assuming that leptin is increased and adiponectin is decreased, in obesity cases. (AU)

FAPESP's process: 10/16911-4 - GENOMIC AND NON GENOMIC ACTION OF TRIIODOTIRONINE (T3) IN THE GENE EXPRESSION AND LEPTIN AND ADIPONECTIN PROTEIN IN CELLS 3T3-L1
Grantee:Celia Regina Nogueira
Support Opportunities: Regular Research Grants