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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Relationship between pemphigus and American tegumentary leishmaniasis: insights from serological and genetic profiles

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Author(s):
Walsh, Priscilla [1] ; Brochado, Maria J. F. [1] ; Vernal, Sebastian [1] ; Machado, Aline R. [1] ; Turatti, Aline [1] ; de Paula, Natalia A. [1] ; Donadi, Eduardo A. [2] ; Roselino, Ana Maria [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Clin Med, Div Dermatol, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Clin Med, Div Immunol, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Transactions of the Royal Society of Tropical Medicine and Hygiene; v. 111, n. 8, p. 345-353, AUG 2017.
Web of Science Citations: 0
Abstract

Background: Antibodies against Leishmania peptides (Lbr-peps) and desmogleins (Dsgs) have been reported in pemphigus foliaceus (PF) and leishmaniasis patients, respectively. We aimed to compare serological and genetic features in a Brazilian region endemic for American tegumentary leishmaniasis (ATL) and pemphigus. Methods: Commercial anti-Dsg ELISA and in-house ELISA with Lbr-peps were used to determine the serological profile, in addition to immunoblotting (IB) and indirect immunofluorescence (IIF) assays. HLA-DRB1 and -DQA1/DQB1 alleles were characterized by PCR combined with sequence-specific oligonucleotide probes (PCR-SSOP). The serological and genetic profiles were compared using 78 PF, 62 pemphigus vulgaris (PV) and 58 ATL patients against 163 and 1592 healthy controls, respectively. Results: Some ATL patients showed positive results for anti-Dsg1 and/or anti-Dsg3 antibodies. They also revealed 130, 160 and/or 230 kDa epidermal peptides in IB. Moreover, some ATL samples exhibited pemphigus or a bullous pemphigoid pattern in IIF. ELISA and IB assays showed Lbr-peps in pemphigus patients. HLA-DQA1{*}01 and -DQA1{*}01:02 were protective and susceptibility alleles for ATL, respectively, but the opposite for pemphigus. Conclusions: Anti-Dsgs in ATL may represent epiphenomena. Anti-Lbr-pep antibodies in pemphigus suggest a previous infection. A differential association of the HLA profile may contribute to the lack of co-association between pemphigus and ATL. (AU)

FAPESP's process: 10/51729-2 - Relation between immunogenetics and environmental factors in a Brazilian epidemiological study of Pemphigus vulgaris and Pemphigus foliaceus
Grantee:Ana Maria Ferreira Roselino
Support Opportunities: Research Projects - Thematic Grants