Cardiac Morphofunctional Characteristics of Transgenic Rats With Overexpression of the Bradykinin B1 Receptor in the Endothelium

Author(s):Show less -

Levy, R. F. ^[1] ; Serra, A. J. ^[2] ; Antonio, E. L. ^[3] ; dos Santos, L. ^[4] ; Bocalini, D. S. ^[5] ; Pesquero, J. B. ^[3] ; Bader, M. ^[6] ; Merino, V. F. ^[7] ; de Oliveira, H. A. ^[2] ; de Arruda Veiga, E. C. ^[3] ; Silva, Jr., J. A. ^[2] ; Tucci, P. J. F. ^[3]

Affiliation

^[1] Univ Fed Paraiba, Joao Pessoa, Paraiba - Brazil

^[2] Univ Nove Julho, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Sao Paulo, SP - Brazil

^[4] Univ Fed Espirito Santo, Vitoria, ES - Brazil

^[5] Univ Sao Judas Tadeu, Sao Paulo, SP - Brazil

^[6] Max Delbruck Ctr Mol Med MDC, Berlin - Germany

^[7] Johns Hopkins Univ, Sch Med, Baltimore, MD - USA

• Document type: Journal article
• Source: Physiological Research; v. 66, n. 6, p. 925-932, 2017.
• Web of Science Citations: 0

Abstract

Our aim was to evaluate whether endothelial overexpressing of the bradykinin B1 receptor could be associated with altered left ventricular and myocardial performance. Echocardiography and hemodynamic were employed to assess left ventricular morphology and function in Sprague Dawley transgenic rats overexpressing the endothelial bradykinin B1 receptor (Tie2B1 rats). The myocardial inotropism was evaluated on papillary muscles contracting in vitro. In Tie2B1 animals, an enlarged left ventricular cavity and lower fractional shortening coupled with a lower rate of pressure change values indicated depressed left ventricular performance. Papillary muscle mechanics revealed that both Tie2B1 and wild-type rat groups had the same contractile capacities under basal conditions; however, in transgenic animals, there was accentuated inotropism due to post-pause potentiation. Following treatment with the Arg(9)-BK agonist, Tie2B1 papillary muscles displayed a reduction in myocardial inotropism. Endothelial B1 receptor overexpression has expanded the LV cavity and worsened its function. There was an exacerbated response of papillary muscle in vitro to a prolonged resting pause, and the use of a B1 receptor agonist impairs myocardial inotropism. (AU)

• FAPESP's process: 09/54225-8 - The pathophysiology of congestive heart failure: from the organ to tile molecules
• Grantee: Paulo Jose Ferreira Tucci
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/11028-9 - The aerobic training method can determine the existence of cardiac remodeling: structural, functional and molecular analyzes of swimming and running experimental models.
• Grantee: Andrey Jorge Serra
• Support Opportunities: Regular Research Grants