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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Simultaneous Production of Anabaenopeptins and Namalides by the Cyanobacterium Nostoc sp CENA543

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Author(s):
Shishido, Tania K. [1] ; Jokela, Jouni [1] ; Fewer, David P. [1] ; Wahlsten, Matti [1] ; Fiore, Marli F. [2] ; Sivonen, Kaarina [1]
Total Authors: 6
Affiliation:
[1] Univ Helsinki, Dept Food & Environm Sci, Viikki Bioctr 1, POB 56, FIN-00014 Helsinki - Finland
[2] Univ Sao Paulo, Ctr Nucl Energy Agr, Ave Centenario 303, BR-13400970 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ACS Chemical Biology; v. 12, n. 11, p. 2746-2755, NOV 2017.
Web of Science Citations: 8
Abstract

Anabaenopeptins are a diverse group of cyclic peptides, which contain an unusual ureido linkage. Namalides are shorter structural homologues of anabaenopeptins, which also contain an ureido linkage. The biosynthetic origins of namalides are unknown despite a strong resemblance to anabaenopeptins. Here, we show the cyanobacterium Nostoc sp. CENA543 strain producing new (nostamide B-E (2, 4, 5, and 6)) and known variants of anabaenopeptins (schizopeptin 791 (1) and anabaenopeptin 807 (3)). Surprisingly, Nostoc sp. CENA543 also produced namalide B (8) and the new namalides D (7), E (9), and F (10) in similar amounts to anabaenopeptins. Analysis of the complete Nostoc sp. CENA543 genome sequence indicates that both anabaenopeptins and namalides are produced by the same biosynthetic pathway through module skipping during biosynthesis. This unique process involves the skipping of two modules present in different nonribosomal peptide synthetases during the namalide biosynthesis. This skipping is an efficient mechanism since both anabaenopeptins and namalides are synthesized in similar amounts by Nostoc sp. CENA543. Consequently, gene skipping may be used to increase and possibly broaden the chemical diversity of related peptides produced by a single biosynthetic gene cluster. Genome mining demonstrated that the anabaenopeptin gene clusters are widespread in cyanobacteria and can also be found in tectomicrobia bacteria. (AU)

FAPESP's process: 13/50425-8 - Biodiversity of cyanobacteria and their bioactive compounds from under-explored Brazilian habitats
Grantee:Marli de Fátima Fiore
Support Opportunities: Regular Research Grants