CCR5-Dependent Homing of T Regulatory Cells to the Tumor Microenvironment Contributes to Skin Squamous Cell Carcinoma Development

Author(s):Show less -

de Oliveira, Carine Ervolino ^[1] ; Gasparoto, Thais Helena ^[2] ; Pinheiro, Claudia Ramos ^[2] ; Amor, Nadia Ghinelli ^[2] ; Sales Nogueira, Maria Renata ^[3] ; Kaneno, Ramon ^[4] ; Garlet, Gustavo Pompermaier ^[2] ; Lara, Vanessa Soares ^[1] ; Silva, Joao Santana ^[5] ; Cavassani, Karen Angelica ^[6] ; Campanelli, Ana Paula ^[2]

Affiliation

^[1] Uni Sao Paulo, Bauru Sch Dent, Dept Stomatol Oral Pathol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Sao Paulo - Brazil

^[3] Lauro de Souza Lima Inst, Hlth State Dept, Dept Res Div, Bauru, SP - Brazil

^[4] Sao Paulo State Univ, Inst Biosci Botucatu, Dept Microbiol & Immunol, Botucatu, SP - Brazil

^[5] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Biochem & Immunol, Ribeirao Preto, SP - Brazil

^[6] Cedars Sinai Med Ctr, Samuel Oschin Comprehens Canc Inst, Los Angeles, CA 90048 - USA

• Document type: Journal article
• Source: MOLECULAR CANCER THERAPEUTICS; v. 16, n. 12, p. 2871-2880, DEC 2017.
• Web of Science Citations: 5

Abstract

Squamous cell carcinoma (SCC) is one of the most common human cancers worldwide. Recent studies show that regulatory T cells (Treg) have a critical role in the modulation of an antitumor immune response, and consequently the SCC development. Because the accumulation of Tregs at the tumor site is, in part, due to selective recruitment through CCR5-and CCR5-associated chemokines, we investigated the role of CCR5 in the SCC development. Our findings showed that CCR5-deficient mice (CCR5KO) were efficient in controlling papilloma's incidence when compared with wild-type mice. Analysis of tumor lesions in wild-type (WT) and CCR5KO mice revealed that lack of CCR5 lead to significant reduction in frequency of Tregs and increased of CD4 T cells into the tumors. Moreover, the adoptive transfer of naturally occurring Tregs CD4(+)CD25(+)CCR5(+), CD4(+)CD25(-) CCR5(+) or CD8(+)CCR5(+) conventional T cells to CCR5KO mice resulted in an increased papilloma incidence. Interestingly, adoptive transfer of WT CD4(+)CD25(+)CCR5(+) cells to CCR5KO mice induced more undifferentiated SCC lesions, characterized by higher infiltration of macrophages and dendritic cells. In this study, we also demonstrated that Treg migration to the tumor microenvironment is mediated by CCR5, and these cells are promoting tumor growth via inhibition of antitumor cells such as cytotoxic CD8(+) T cells. Our findings reinforce the therapeutic potential of CCR5 inhibition for cancer treatment, and indicate an attractive approach for SCC treatment. (C) 2017 AACR. (AU)

• FAPESP's process: 12/15331-0 - Analysis of CCR5-dependent Regulatory T cell migration: correlation with SCC development
• Grantee: Ana Paula Campanelli
• Support Opportunities: Regular Research Grants