Poly(allylamine hydrochloride) (PAH) and Bovine Serum Albumin (BSA) Protein Nanostructured as Layer-by-Layer Thin Films

Author(s)

Lima de Sa, Antonio Wellington ^[1] ; de Lemos, Hugo Gajardoni ^[1] ; Pereira-da-Silva, Marcelo A. ^{[2, 3]} ; Venancio, Everaldo Carlos ^[1]

Affiliation

^[1] Univ Fed Abc, Ctr Engn Modelagem & Ciencias Sociais Aplicadas C, Ave Estados 5001, BR-09210580 Santo Andre, SP - Brazil

^[2] Ctr Univ Cent Paulista UNICEP, BR-13563470 Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, IFSC, BR-13560250 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Journal of Nanoscience and Nanotechnology; v. 18, n. 6, p. 3908-3915, JUN 2018.
• Web of Science Citations: 1

Abstract

In this work it was studied the production of nanostructured thin films containing alternating layers of poly(allylamine hydrochloride) (PAH) and bovine serum albumin protein (BSA). It was studied the adsorption processes of these materials onto solid surfaces, which included adsorption kinetics and film growth studies by using UV-Vis spectroscopy. The film morphology, thickness and roughness were studied by using atomic force microscopy (AFM). The AFM results showed that the film presented a globular-like surface morphology and a thickness within the nanoscale, which could be controlled by the number of deposited layers. The roughness and thickness of the film depend on the number of deposited bilayers, where the film roughness increased for films with a number of bilayers up to five, after which the film roughness remained almost constant. This is an indicative that the adsorption and growth processes might occur under two different mechanisms, first under the influence of the substrate (films containing up to five bilayers of PAH/BSA), and second under the influence of the film previously deposited (films containing more than five bilayers of PAH/BSA), respectively. (AU)

• FAPESP's process: 11/16615-9 - Development of Electrocnic organic polymer-based nanocomposites
• Grantee: Hugo Gajardoni de Lemos
• Support Opportunities: Scholarships in Brazil - Master