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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Crystal structure and insights into the oligomeric state of UDP-glucose pyrophosphorylase from sugarcane

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Cotrim, Camila A. [1, 2, 3] ; Soares, Jose Sergio M. [4] ; Kobe, Bostjan [1, 2] ; Menossi, Marcelo [4]
Total Authors: 4
[1] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld - Australia
[2] Univ Queensland, Australia Infect Dis Res Ctr, Brisbane, Qld - Australia
[3] Griffith Univ, Griffith Inst Drug Discovery, Brisbane, Qld - Australia
[4] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao Microbiol & Imunol, Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PLoS One; v. 13, n. 3 MAR 1 2018.
Web of Science Citations: 3

UDP-glucose pyrophosphorylase (UGPase) is found in all organisms and catalyses the formation of UDP-glucose. In sugarcane, UDP-glucose is a branch-point in the carbon channelling into other carbohydrates, such as sucrose and cellulose, which are the major factors for sugarcane productivity. In most plants, UGPase has been described to be enzymatically active in the monomeric form, while in human and yeast, homo-octamers represent the active form of the protein. Here, we present the crystal structure of UGPase from sugarcane (ScUGPase-1) at resolution of 2.0 angstrom. The crystals of ScUGPase-1 reveal the presence of two molecules in the asymmetric unit and the multi-angle light scattering analysis shows that ScUGPase-1 forms a mixture of species ranging from monomers to larger oligomers in solution, suggesting similarities with the orthologs from yeast and human. (AU)

FAPESP's process: 13/15576-5 - Molecular approaches to study the relationship between ethylene and sugarcane ripening
Grantee:Marcelo Menossi Teixeira
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 08/06767-3 - Structural and functional characterization of enzymes involved in sucrose biosynthesis and accumulation in Sugarcane
Grantee:José Sérgio de Macedo Soares
Support Opportunities: Scholarships in Brazil - Doctorate