Amperometric determination of myo-inositol by using a glassy carbon electrode modified with molecularly imprinted polypyrrole, reduced graphene oxide and nickel nanoparticles

Author(s)

Beluomini, Maisa Azevedo ^[1] ; da Silva, Jose Luiz ^[1] ; Stradiotto, Nelson Ramos ^{[1, 2, 3]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Chem, BR-14800060 Sao Paulo - Brazil

^[2] Sao Paulo State Univ UNESP, Bioenergy Res Inst, BR-13500230 Sao Paulo - Brazil

^[3] UFF, Sch Met & Ind Engn, BR-27255125 Rio De Janeiro - Brazil

• Document type: Journal article
• Source: Microchimica Acta; v. 185, n. 3 MAR 2018.
• Web of Science Citations: 4

Abstract

This paper reports on the development of an amperometric method for the determination of myo-inositol. The method involves coating of a glassy carbon electrode (GCE) with a molecularly imprinted polymer (MIP) and reduced graphene oxide (RGO) that was modified with nickel nanoparticles (NiNPs). The MIP was prepared by electropolymerization of pyrrole on the surface of the GCE in the presence of myo-inositol molecules. The construction steps of the modified electrode were monitored via cyclic voltammetry, atomic force microscopy, scanning electron microscopy and X-ray Photoelectron Spectroscopy. The results were evaluated using differential pulse voltammetry, in which hexacyanoferrate was used as an electrochemically active probe. Under optimized experimental conditions, the imprint-modified GCE has a linear response in the 1.0 x 10(-10) mol L-1 to 1.0 x 10(-8) mol L-1 concentration range, with a 7.6 x 10(-11) mol L-1 detection limit and an electrochemical sensitivity of 4.5 mu A.cm(-2) mu mol(-1). The method showed improved selectivity even in the presence of molecules with similar chemical structure. The GCE modified was successfully applied to the determination of myo-inositol in sugarcane vinasse where it yielded recoveries that ranged from 95 to 102%. (AU)

• FAPESP's process: 14/23846-5 - New methods for determination of carboxylic acids, amino acids and polyols in vinasse of the manufacturing process of ethanol
• Grantee: Nelson Ramos Stradiotto
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants