Development of an advanced electroanalytical methodology for quantification of the hormones 17²-estradiol and 17±-etinilestradiol in supply water samples using electrodes modified with graphene, gold nanoparticles and quantum dots

• Grant number: 16/12759-0
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2016
• End date: November 30, 2018
• Field of knowledge: Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment
• Principal Investigator: Sergio Antonio Spinola Machado
• Grantee: Sergio Antonio Spinola Machado
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

This project aims to develop an advanced electroanalytical methodology for quantifying hormones (17²-estradiol and 17±-ethinylestradiol) in supply water samples. For this, an electrochemical sensor of glassy carbon will be modified by coating the surface with oxidized graphene (GO) nanocomposites with gold nanoparticles (npAu) and CdTe/CdS (QDs - quantum dots). This modified electrode (GO/Np-Au/QDs) will be characterized by techniques such as atomic force microscopy (AFM), scanning electron microscopy (SEM), transmission microscopy, spectroscopic diffraction of X-rays and X-ray energy dispersive (EDX), as well as by electrochemical techniques (cyclic voltammetry, square wave voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy - IEE). After characterizing the surface, the developed electrode will be applied to the study of the electrochemical behavior of hormones in different support electrolytes, different concentrations, different electrochemical parameters and pHs. The sensor developed and optimized here will enable increased sensitivity and consequently the determination of hormones 17²-estradiol and 17±-ethinylestradiol at low detection limits. With this, it will be used in drinking water samples collected in the São Carlos Water Treatment Plant, before and after the water purification procedure. The detection of such contaminants will occur via direct electrochemical oxidation/reduction at the GO/Np-Au/QDs sensor surface for both 17²-estradiol and 17±-ethinyl estradiol. The results of this project will be an electroanalytic device with characteristics as accuracy, sensitivity, potential for miniaturization and possibility of "in situ " and in real time applications (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RAYMUNDO-PEREIRA, PAULO A.; CAMPOS, ANDERSON M.; VICENTINI, FERNANDO C.; JANEGITZ, BRUNO C.; MENDONCA, CAMILA D.; FURINI, LEONARDO N.; BOAS, NAIZA V.; CALEGARO, MARCELO L.; CONSTANTINO, CARLOS J. L.; MACHADO, SERGIO A. S.; et al. Sensitive detection of estriol hormone in creek water using a sensor platform based on carbon black and silver nanoparticles. Talanta, v. 174, p. 652-659, NOV 1 2017. (16/01919-6, 14/05197-0, 13/14262-7, 16/12759-0, 15/19099-2, 12/17689-9)

RAYMUNDO-PEREIRA, PAULO A.; CAMPOS, ANDERSON M.; MENDONCA, CAMILA D.; CALEGARO, MARCELO L.; MACHADO, SERGIO A. S.; OLIVEIRA, JR., OSVALDO N.. Printex 6L Carbon Nanoballs used in Electrochemical Sensors for Simultaneous Detection of Emerging Pollutants Hydroquinone and Paracetamol. SENSORS AND ACTUATORS B-CHEMICAL, v. 252, p. 165-174, NOV 2017. (16/01919-6, 14/05197-0, 13/14262-7, 16/12759-0, 12/17689-9)

CAMPOS, ANDERSON M.; RAYMUNDO-PEREIRA, PAULO A.; MENDONCA, CAMILA D.; CALEGARO, MARCELO L.; MACHADO, SERGIO A. S.; OLIVEIRA, JR., OSVALDO N.. Size Control of Carbon Spherical Shells for Sensitive Detection of Paracetamol in Sweat, Saliva, and Urine. ACS APPLIED NANO MATERIALS, v. 1, n. 2, p. 654-661, FEB 2018. (16/01919-6, 14/05197-0, 13/14262-7, 16/12759-0, 12/17689-9)

BOTT-NETO, JOSE L.; MARTINS, THIAGO S.; MACHADO, SERGIO A. S.; TICIANELLI, EDSON A.. Electrocatalytic Oxidation of Methanol, Ethanol, and Glycerol on Ni(OH)(2) Nanoparticles Encapsulated with Poly[Ni(salen)] Film. ACS APPLIED MATERIALS & INTERFACES, v. 11, n. 34, p. 30810-30818, AUG 28 2019. (16/12759-0, 13/16930-7)

MARTINS, THIAGO S.; BOTT-NETO, JOSE L.; RAYMUNDO-PEREIRA, PAULO A.; TICIANELLI, EDSON A.; MACHADO, SERGIO A. S.. An electrochemical furosemide sensor based on pencil graphite surface modified with polymer film Ni-salen and Ni(OH)(2)/C nanoparticles. SENSORS AND ACTUATORS B-CHEMICAL, v. 276, p. 378-387, DEC 10 2018. (16/01919-6, 16/12759-0, 13/16930-7)

RAYMUNDO-PEREIRA, PAULO A.; GOMES, NATHALIA O.; MACHADO, SERGIO A. S.; OLIVEIRA, JR., OSVALDO N.. Simultaneous, ultrasensitive detection of hydroquinone, paracetamol and estradiol for quality control of tap water with a simple electrochemical method. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 848, SEP 1 2019. (16/12759-0, 13/14262-7, 16/01919-6, 19/01777-5)