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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

CCR5 Delta 32 (rs333) polymorphism is associated with decreased risk of chronic and aggressive periodontitis: A case-control analysis based in disease resistance and susceptibility phenotypes

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Cavalla, Franco [1, 2] ; Biguetti, Claudia C. [1] ; Dionisio, Thiago J. [1] ; Azevedo, Michelle C. S. [1] ; Martins, Jr., Walter [3] ; Santos, Carlos F. [1] ; Trombone, Ana Paula F. [4] ; Silva, Renato M. [5] ; Letra, Ariadne [5] ; Garlet, Gustavo P. [1]
Total Authors: 10
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Sao Paulo - Brazil
[2] Univ Chile, Sch Dent, Dept Conservat Dent, Santiago - Chile
[3] Univ Ribeirao Preto, Sch Dent, Dept Periodont, Ribeirao Preto - Brazil
[4] Univ Sagrado Coracao, Dept Biol & Allied Hlth Sci, Bauru - Brazil
[5] Univ Texas Sch Dent Houston, Dept Endodont, Houston, TX - USA
Total Affiliations: 5
Document type: Journal article
Source: CYTOKINE; v. 103, p. 142-149, MAR 2018.
Web of Science Citations: 5

Chronic and aggressive periodontitis are infectious diseases characterized by the irreversible destruction of periodontal tissues, which is mediated by the host inflammatory immune response triggered by periodontal infection. The chemokine receptor CCR5 play an important role in disease pathogenesis, contributing to pro inflammatory response and osteoclastogenesis. CCR5 Delta 32 (rs333) is a loss-of-function mutation in the CCR5 gene, which can potentially modulate the host response and, consequently periodontitis outcome. Thus, we investigated the effect of the CCR5 Delta 32 mutation over the risk to suffer periodontitis in a cohort of Brazilian patients (total N = 699), representative of disease susceptibility (chronic periodontitis, N = 197; and aggressive periodontitis, N-= 91) or resistance (chronic gingivitis, N = 193) phenotypes, and healthy subjects (N = 218). Additionally, we assayed the influence of CCR5 Delta 32 in the expression of the biomarkers TNFa, IL-10, IL-6, IFN-y and T-bet, and key periodontal pathogens P. gingivalis, T. forsythia, and T. denticola. In the association analysis of resistant versus susceptible subjects, CCR5 Delta 32 mutant allele-carriers proved significantly protected against chronic (OR 0.49; 95% CI 0.29-0.83; p-value 0.01) and aggressive (OR 0.46; 95% CI 0.22-0.94; p-value 0.03) periodontitis. Further, heterozygous subjects exhibited significantly decreased expression of TNFa in periodontal tissues, pointing to a functional effect of the mutation in periodontal tissues during the progression of the disease. Conversely, no significant changes were observed in the presence or quantity of the periodontal pathogens P. gingivalis, T. forsythia, and T. denticola in the subgingival biofilm that could be attributable to the mutant genotype. (AU)

FAPESP's process: 14/03276-0 - Influence of genetic polymorphisms in the colonization/recolonization patterns in chronic periodontitis patients
Grantee:Ian Franco Cavalla Ruiz
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/17886-4 - Influence of genetic polymorphisms in the patterns of bacterial colonization and recolonization in chornic periodontitis patients
Grantee:Gustavo Pompermaier Garlet
Support Opportunities: Regular Research Grants