Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models

Author(s):Show less -

Bottini, Massimo ^{[1, 2]} ; Mebarek, Saida ^{[3, 4, 5, 6, 7]} ; Anderson, Karen L. ^[1] ; Strzelecka-Kiliszek, Agnieszka ^[8] ; Bozycki, Lukasz ^[8] ; Sper Simao, Ana Maria ^[9] ; Bolean, Mayte ^[9] ; Ciancaglini, Pietro ^[9] ; Pikula, Joanna Bandorowicz ^[8] ; Pikula, Slawomir ^[8] ; Magne, David ^{[3, 4, 5, 6, 7]} ; Volkmann, Niels ^[1] ; Hanein, Dorit ^[1] ; Millan, Jose Luis ^[1] ; Buchet, Rene ^{[3, 4, 5, 6, 7]}

Affiliation

^[1] Sanford Burnham Prebys Med Discovery Inst, La Jolla, CA 92037 - USA

^[2] Univ Roma Tor Vergata, Dept Expt Med & Surg, I-00133 Rome - Italy

^[3] Univ Lyon, F-69622 Villeurbanne - France

^[4] Univ Lyon 1, UFR Chim Biochim, F-69622 Villeurbanne - France

^[5] CNRS, ICBMS, UMR 5246, F-69622 Villeurbanne - France

^[6] INSA, F-69622 Villeurbanne - France

^[7] CPE, F-69622 Villeurbanne - France

^[8] Polish Acad Sci, Dept Biochem, Nencki Inst Expt Biol, PL-02093 Warsaw - Poland

^[9] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao, BR-14040901 Ribeirao Preto, SP - Brazil

• Document type: Review article
• Source: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS; v. 1862, n. 3, p. 532-546, MAR 2018.
• Web of Science Citations: 25

Abstract

Background: Matrix vesicles (MVs) are released from hypertrophic chondrocytes and from mature osteoblasts, the cells responsible for endochondral and membranous ossification. Under pathological conditions, they can also be released from cells of non-skeletal tissues such as vascular smooth muscle cells. MVs are extracellular vesicles of approximately 100-300 nm diameter harboring the biochemical machinery needed to induce mineralization. Scope of the review: The review comprehensively delineates our current knowledge of MV biology and highlights open questions aiming to stimulate further research. The review is constructed as a series of questions addressing issues of MVs ranging from their biogenesis and functions, to biomimetic models. It critically evaluates experimental data including their isolation and characterization methods, like lipidomics, proteomics, transmission electron microscopy, atomic force microscopy and proteoliposome models mimicking MVs. Major conclusions: MVs have a relatively well-defined function as initiators of mineralization. They bind to collagen and their composition reflects the composition of lipid rafts. We call attention to the as yet unclear mechanisms leading to the biogenesis of MVs, and how minerals form and when they are formed. We discuss the prospects of employing upcoming experimental models to deepen our understanding of MV-mediated mineralization and mineralization disorders such as the use of reconstituted lipid vesicles, proteoliposomes and, native sample preparations and high-resolution technologies. (AU)

• FAPESP's process: 14/11941-3 - Are collagen and Annexin V responsible for the control in the biomineralization process?
• Grantee: Pietro Ciancaglini
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/21236-0 - Extracellular matrix vesicles (MVs) mimetic systems to study the regulation of the biomineralization process: proteoliposomes containing NPP1 and Annexin V
• Grantee: Pietro Ciancaglini
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/00371-1 - Are the interactions between collagen and proteins/enzymes present in the matriz vesicles responsible for the control in the biomineralization process?
• Grantee: Maytê Bolean Correia
• Support Opportunities: Scholarships in Brazil - Post-Doctoral