Guidelines for DNA barcoding of coralline algae, focusing on Lithophylloideae (Corallinales) from Brazil

Author(s)

Torrano-Silva, Beatriz N. ^[1] ; Vieira, Bruno R. ^[1] ; Riosmena-Rodriguez, Rafael ^[2] ; Oliveira, Mariana C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Bot, Inst Biociencias, Rua Matao, 277, Cidade Univ, BR-05508090 Sao Paulo, SP - Brazil

^[2] Univ Autonoma Baja California Sur, Dept Biol Marina, Programa Invest Bot Marina, Apartado Postal 19-B, La Paz 23080, Bcs - Mexico

• Document type: Journal article
• Source: Botanica Marina; v. 61, n. 2, p. 127-140, APR 2018.
• Web of Science Citations: 3

Abstract

Multiple-marker (COI-5P, UPA, psbA and rbcL-3P) and two algorithmic approaches {[}automatic barcode gap discovery (ABGD) and Poisson tree process (PTP)] were used for species delimitation of Lithophylloideae in Brazil. The integrative approach was mostly congruent between markers and algorithmic methods of species delimitation, suggesting the occurrence of 24 species. Based on morphology and molecular data, Amphiroa rigida, Amphiroa vanbosseae, Lithophyllum atlanticum, Lithophyllum kaiseri, Lithophyllum margaritae, Titanoderma pustulatum, Titanoderma prototypum and Paulsilvella huveorum, which were previously reported for Brazil, are confirmed in this work. Six new species are distinguished by both molecular and morphological traits, and they are provisionally named as Amphiroa sp. 1, Amphiroa sp. 2, Amphiroa sp. 3, Lithophyllum sp. 1, Lithophyllum sp. 2 and Lithophyllum sp. 3. Another 10 species are cryptic and cannot be distinguished based on traditionally used morphological traits. These include Amphiroa sp. 4, Lithophyllum sp. 4, three species that are morphologically named under Amphiroa beauvoisii, and six that share the morphology described for Amphiroa fragilissima. All four markers used were useful for species delimitation. However, a combination of practical aspects and levels of intra-and interspecific divergence values led us to propose the use of rbcL-3P as a standard DNA barcode marker for the Corallinales. (AU)

• FAPESP's process: 10/19234-3 - Taxonomy and phylogeny from the subfamily Lithophylloideae in Brazil.
• Grantee: Beatriz Nogueira Torrano da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/11833-3 - Diversity and phylogeny of red algae (Rhodophyta) of economic and ecological importance
• Grantee: Mariana Cabral de Oliveira
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants