Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis

Author(s)

Hernandez-Montelongo, J. ^{[1, 2, 3]} ; Corrales Urena, Y. R. ^[4] ; Machado, D. ^[5] ; Lancelloti, M. ^[5] ; Pinheiro, M. P. ^[6] ; Rischka, K. ^[6] ; Lisboa-Filho, P. N. ^[7] ; Cotta, M. A. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Inst Fis Gleb Wataghin, Dept Fis Aplicada, BR-13083859 Campinas, SP - Brazil

^[2] Univ Catolica Temuco, Fac Ingn, Dept Ciencias Matemat & Fis, Temuco 4813302 - Chile

^[3] Univ Catolica Temuco, Fac Ingn, Nucleo Invest Bioprod & Mat Avanzados BioMa, Temuco 4781312 - Chile

^[4] Ctr Natl Alta Tecnol, Lab Nacl Nanotecnol, San Jose 11741200 - Costa Rica

^[5] Univ Estadual Campinas, Fac Ciencias Farmaceut, BR-13083871 Campinas, SP - Brazil

^[6] Fraunhofer Inst Mfg Technol & Adv Mat, D-28359 Bremen - Germany

^[7] Univ Estadual Paulista, Fac Ciencias, Dept Fis, BR-17033360 Bauru - Brazil

• Document type: Journal article
• Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 164, p. 370-378, APR 1 2018.
• Web of Science Citations: 0

Abstract

Staphylococcus epidermidis is a gram-positive bacterium, and one of the most prevalent causes of nosocomial infections due to its strong ability to form biofilms on catheters and surgical implants. Here we explore the antimicrobial properties of Tet-124 peptides, which are part of the innate defense against different multicellular organisms in nature. Two different Tet-124 peptides were immobilized on a polyethylenimine (PEI) film to determine their impact on the antimicrobial properties: KLWWMIRRW (Tet-124), which contains only natural amino acids, and KLWWMIRRWG-(F-Br)-G (F-Br- 4-Bromophenylalanine), a modified Tet-124 sequence with the addition of an unnatural amino acid. The immobilization was obtained as a result of the electrostatic interaction between PEI amino groups and the C-terminal carboxylic groups of tryptophan and glycine amino acids of Tet-124 and Tet-124-Br peptides, respectively. The process was monitored and studied by water contact angle, Atomic Force Microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS) and Quartz Crystal Microbalance with Dissipation (QCM-D) measurements. The antibacterial effect of our samples against S. epidermis was evaluated by the spread plate counting method, and cytotoxicity was tested using fibroblast cultures. Our results indicate the feasibility to immobilize electrostatically both Tet-124 peptides for biomedical applications. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 15/16611-4 - III-V semiconductor nanowires: synthesis studies for biology applications
• Grantee: Mônica Alonso Cotta
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/14888-3 - Adhesion behavior of Pseudomonas aeruginosa and Staphylococcus aureus bacteria on nanostructured films based on chitosan: studies at the micro and nanoscales.
• Grantee: Jesús Jacobo Hernández Montelongo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral