JMJD1A, H3K9me1, H3K9me2 and ADM expression as prognostic markers in oral and oropharyngeal squamous cell carcinoma

Author(s):Show less -

Maia, Lucas de Lima ^[1] ; Peterle, Gabriela Tonini ^[1] ; dos Santos, Marcelo ^[2] ; Trivilin, Leonardo Oliveira ^[1] ; Mendes, Suzanny Oliveira ^[1] ; de Oliveira, Mayara Mota ^[1] ; dos Santos, Joaquim Gasparini ^[1] ; Stur, Elaine ^[1] ; Agostini, Lidiane Pignaton ^[1] ; Monteiro da Silva Couto, Cinthia Vidal ^[1] ; Dalbo, Juliana ^[1] ; Evangelista Monteiro de Assisi, Aricia Leone ^[1] ; Archanjo, Anderson Barros ^[1] ; Da Cunha Mercante, Ana Maria ^[3] ; Mendoza Lopez, Rossana Veronica ^[4] ; Nunes, Fabio Daumas ^[5] ; de Carvalho, Marcos Brasilino ^[6] ; Tajara, Eloiza Helena ^[7] ; Louro, Luri Drumond ^[1] ; Alvares-da-Silva, Adriana Madeira ^[1]

Affiliation

^[1] Univ Fed Espirito Santo, Programa Posgrad Biotecnol, Vitoria, ES - Brazil

^[2] Univ Fed Rio Grande do Norte, Escola Multicampi Ciencias Med Rio Grande Norte, Caico, RN - Brazil

^[3] Hosp Heliopolis, Dept Anat Patol, Sao Paulo - Brazil

^[4] Inst Canc Estado Sao Paulo, Ctr Invest Translac Oncol, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Dept Patol Bucal, Fac Odontol, Sao Paulo - Brazil

^[6] Hosp Heliopolis, Lab Biol Mol, Sao Paulo - Brazil

^[7] Fac Med, Dept Biol Mol, Sao Paulo - Brazil

• Document type: Journal article
• Source: PLoS One; v. 13, n. 3 MAR 28 2018.
• Web of Science Citations: 4

Abstract

Aims Jumonji Domain-Containing 1 A (JMJD1A) protein promotes demethylation of histones, especially at lysin-9 of di-methylated histone H3 (H3K9me2) or mono-methylated (H3K9me1). Increased levels of H3 histone methylation at lysin-9 (H3K9) is related to tumor suppressor gene silencing. JMJD1A gene target Adrenomeduline (ADM) has shown to promote cell growth and tumorigenesis. JMJD1A and ADM expression, as well as H3K9 methylation level have been related with development risk and prognosis of several tumor types. Methods and results We aimed to evaluate JMJD1A, ADM, H3K9me1 and H3K9me2expression in paraffin embedded tissue microarrays from 84 oral and oropharyngeal squamous cell carcinoma samples through immunohistochemistry analysis. Our results showed that nuclear JMJD1A expression was related to lymph node metastasis risk. In addition, JMJD1A cytoplasmic expression was an independent risk marker for advanced tumor stages. H3K9me1 cytoplasmic expression was associated with reduced disease-specific death risk. Furthermore, high H3K9me2 nuclear expression was associated with worse specific-disease and disease-free survival. Finally, high ADM cytoplasmic expression was an independent marker of lymph node metastasis risk. Conclusion JMJD1A, H3K9me1/2 and ADM expression may be predictor markers of progression and prognosis in oral and oropharynx cancer patients, as well as putative therapeutic targets. (AU)

• FAPESP's process: 04/12054-9 - Markers of aggressive behavior in head and neck tumors
• Grantee: Eloiza Helena Tajara da Silva
• Support Opportunities: Research Projects - Thematic Grants