The inhibition of 5-Lipoxygenase (5-LO) products leukotriene B4 (LTB4) and cysteinyl leukotrienes (cysLTs) modulates the inflammatory response and improves cutaneous wound healing

Author(s):Show less -

Guimaraes, Francielle Rodrigues ^{[1, 2]} ; Sales-Campos, Helioswilton ^{[1, 2]} ; Nardini, Viviani ^[2] ; da Costa, Thiago Alvares ^[1] ; Costa Fonseca, Monique Thais ^{[3, 1]} ; Rodrigues Junior, Virmondes ^[1] ; Sorgi, Carlos Arterio ^[2] ; da Silva, Joao Santana ^[3] ; Lazo Chica, Javier Emilio ^[1] ; Faccioli, Lucia Helena ^[2] ; de Barros Cardoso, Cristina Ribeiro ^{[1, 2]}

Affiliation

^[1] Univ Fed Triangulo Mineiro, Inst Ciencias Biol & Nat, Uberaba, MG - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Clinical Immunology; v. 190, p. 74-83, MAY 2018.
• Web of Science Citations: 3

Abstract

To analyze the participation of the enzyme 5-lipoxygenase (5-LO) in skin repair, WT wounds were compared to those in 5-LO deficient mice (5-LO-/-), which presented faster closure and reduced inflammatory infiltrate in the skin, together with increased CD4 regulatory T cells markers in the draining lymph nodes. The 5-LO-/- wounds also had diminished TNF-alpha, CCL11, CCL7, CCL2, CXCL9, CCRI and CXCR2 mRNA expression in the lesions, besides differential extracellular matrix remodeling. Furthermore, when cysteinyl leukotriene (cysLT) and leukotriene (LTB4) receptors were antagonized in WT mice, there was a remarkable reduction in TNF-alpha expression and faster skin healing, similarly to the findings in 5-LO-/- animals. Finally, our results suggested that 5-LO products, in special cysLT and LTB4, underline skin inflammation that follows skin injury and their neutralization may be an important strategy to improve cutaneous healing. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 10/20162-7 - The role of hypothalamus-pituitary-adrenal (HPA) axis and exogenous glucocorticoids in the modulation of immune response in inflammatory bowel disease
• Grantee: Cristina Ribeiro de Barros Cardoso
• Support Opportunities: Research Grants - Young Investigators Grants