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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular Signatures of High-Grade Cervical Lesions

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Author(s):
Porcari, Andreia M. [1] ; Negrao, Fernanda [1] ; Tripodi, Guilherme Lucas [1] ; Pitta, Denise Rocha [2] ; Campos, Elisabete Aparecida [2] ; Montis, Douglas Munhoz [2] ; Martins, Aline M. A. [3] ; Eberlin, Marcos N. [1] ; Derchain, Sophie F. M. [2]
Total Authors: 9
Affiliation:
[1] Univ Estadual Campinas, UNICAMP SP, Dept Chem, Thomson Mass Spectrometry Lab, Campinas, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP SP, Dept Obstet & Gynecol, Campinas, SP - Brazil
[3] Univ Brasilia, Med Coll, LBPQ, Brazilian Ctr Prot Res, Brasilia, DF - Brazil
Total Affiliations: 3
Document type: Journal article
Source: FRONTIERS IN ONCOLOGY; v. 8, APR 12 2018.
Web of Science Citations: 1
Abstract

Cervical cancer is the fourth most common neoplasia in women and the infection with human papilloma virus (HPV) is its necessary cause. Screening methods, currently based on cytology and HPV DNA tests, display low specificity/sensitivity, reducing the efficacy of cervical cancer screening programs. Herein, molecular signatures of cervical cytologic specimens revealed by liquid chromatography-mass spectrometry (LC-MS), were tested in their ability to provide a metabolomic screening for cervical cancer. These molecules were tested whether they could clinically differentiate insignificant HPV infections from precancerous lesions. For that, high-grade squamous intraepithelial lesions (HSIL)related metabolites were compared to those of no cervical lesions in women with and without HPV infection. Samples were collected from women diagnosed with normal cervix (N = 40) and from those detected with HSIL from cytology and colposcopy (N = 40). Liquid-based cytology diagnosis, DNA HPV-detection test, and LC-MS analysis were carried out for all the samples. The same sample, in a customized collection medium, could be used for all the diagnostic techniques employed here. The metabolomic profile of cervical cancer provided by LC-MS was found to indicate unique molecular signatures for HSIL, being two ceramides and a sphingosine metabolite. These molecules occurred independently of women's HPV status and could be related to the pre-neoplastic phenotype. Statistical models based on such findings could correctly discriminate and classify HSIL and no cervical lesion women. The results showcase the potential of LC-MS as an emerging technology for clinical use in cervical cancer screening, although further validation with a larger sample set is still necessary. (AU)

FAPESP's process: 16/11517-2 - Cutaneous leishmaniasis biomarkers candidates search and identification through mass spectrometry
Grantee:Fernanda Negrão Silva
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)