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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Early Stages of XY Sex Chromosomes Differentiation in the Fish Hoplias malabaricus (Characiformes. Erythrinidae) Revealed by DNA Repeats Accumulation

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Author(s):
de Freitas, Natalia Lourenco [1] ; Hamid Al-Rikabi, Ahmed Basheer [1, 2] ; Carlos Bertollo, Luiz Antonio [1] ; Ezaz, Tariq [3] ; Yano, Cassia Fernando [1] ; de Oliveira, Ezequiel Aguiar [1] ; Hatanaka, Terumi [1] ; Cioffi, Marcelo de Bello [1]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP - Brazil
[2] Friedrich Schiller Univ, Jena Univ Hosp, Inst Human Genet, Kollegiengasse 10, D-07743 Jena - Germany
[3] Univ Canberra, Inst Appl Ecol, Canberra, ACT - Australia
Total Affiliations: 3
Document type: Journal article
Source: CURRENT GENOMICS; v. 19, n. 3, p. 216-226, 2018.
Web of Science Citations: 9
Abstract

Background: Species with `young' or nascent sex chromosomes provide unique opportunities to understand early evolutionary mechanisms (e.g. accumulation of repetitive sequences, cessation of recombination and gene loss) that drive the evolution of sex chromosomes. Among vertebrates, fishes exhibit highly diverse and a wide spectrum of sex-determining mechanisms and sex chromosomes, ranging from cryptic to highly differentiated ones, as well as, from simple to multiple sex chromosome systems. Such variability in sex chromosome morphology and composition not only exists within closely related taxa, but often within races/populations of the same species. Inside this context, the wolf fish Hoplias malabaricus offers opportunity to investigate the evolution of morphologically variable sex chromosomes within a species complex, as homomorphic to highly differentiated sex chromosome systems occur among its different karyomorphs. Materials \& Methods: To discover various evolutionary stages of sex chromosomes and to compare their sequence composition among the wolf fish's karyomorphs, we applied multipronged molecular cytogenetic approaches, including C-banding, repetitive DNAs mapping, Comparative Genomic Hybridization (CGH) and Whole Chromosomal Painting (WCP). Our study was able to characterize a cryptically differentiated XX/XY sex chromosome system in the karyomorph F of this species. Conclusion: The Y chromosome was clearly identified by an interstitial heterochromatic block on the short arms, primarily composed of microsatellite motifs and retrotransposons. Additionally, CGH also identified a male specific chromosome region in the same chromosomal location, implying that the accumulation of these repeats may have initiated the Y chromosome differentiation, as well as played a critical role towards the evolution and differentiation of sex chromosomes in various karyomorphs of this species. (AU)

FAPESP's process: 14/22532-7 - Karyotype and sex chromosomes evolution in neotropical fishes
Grantee:Luiz Antonio Carlos Bertollo
Support type: Regular Research Grants