Proteomic Mapping of Dental Enamel Matrix from Inbred Mouse Strains: Unraveling Potential New Players in Enamel

Author(s)

Leite, Aline Lima ^{[1, 2]} ; Fernandes, Mileni Silva ^[2] ; Charone, Senda ^[1] ; Whitford, Gary Milton ^[3] ; Everett, Eric T. ^{[4, 5]} ; Rabelo Buzalaf, Marilia Afonso ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Dent Sch, Dept Biol Sci, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Genet & Mol Biol, Sao Carlos, SP - Brazil

^[3] Georgia Regents Univ, Coll Dent Med, Dept Oral Biol, Augusta, GA - USA

^[4] Univ North Carolina Chapel Hill, Dept Pediat Dent, Chapel Hill, NC - USA

^[5] Univ North Carolina Chapel Hill, Carolina Ctr Genome Sci, Chapel Hill, NC - USA

• Document type: Journal article
• Source: Caries Research; v. 52, n. 1-2, p. 78-87, 2018.
• Web of Science Citations: 2

Abstract

Enamel formation is a complex 2-step process by which proteins are secreted to form an extracellular matrix, followed by massive protein degradation and subsequent mineralization. Excessive systemic exposure to fluoride can disrupt this process and lead to a condition known as dental fluorosis. The genetic background influences the responses of mineralized tissues to fluoride, such as dental fluorosis, observed in A/J and 129P3/J mice. The aim of the present study was to map the protein profile of enamel matrix from A/J and 129P3/J strains. Enamel matrix samples were obtained from A/J and 129P3/J mice and analyzed by 2-dimensional electrophoresis and liquid chromatography coupled with mass spectrometry. A total of 120 proteins were identified, and 7 of them were classified as putative uncharacterized proteins and analyzed in silico for structural and functional characterization. An interesting finding was the possibility of the uncharacterized sequence Q8BIS2 being an enzyme involved in the degradation of matrix proteins. Thus, the results provide a comprehensive view of the structure and function for putative uncharacterized proteins found in the enamel matrix that could help to elucidate the mechanisms involved in enamel biomineralization and genetic susceptibility to dental fluorosis. (C) 2018 S. Karger AG, Basel (AU)

• FAPESP's process: 08/03489-2 - Proteomic analysis of the enamel matrix of mice susceptible or resistant to dental fluorosis, chronically exposed to different levels of fluoride from the drinking water
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Regular Research Grants