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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Large-scale evaluation of a rapid diagnostic test for diarrhea caused by enterotoxigenic Escherichia coli targeting the heat-labile toxin

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Author(s):
Henrique, Camila [1] ; Caetano, Bruna A. [1] ; Mitsunari, Thais [1] ; dos Santos, Luis Fernando [2] ; Piazza, Roxane M. F. [1] ; Rocha, Leticia B. [1]
Total Authors: 6
Affiliation:
[1] Inst Butantan, Lab Bacteriol, Av Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil
[2] Adolfo Lutz Inst, Ctr Bacteriol, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Microbiological Methods; v. 144, p. 125-127, JAN 2018.
Web of Science Citations: 0
Abstract

We standardized an immunochromatographic test (IC) for heat-labile toxin I (LT-I) detection using LT-I antibodies and a specific platform containing the apparatus for application, assembly and cutting. IC detected as little as 62.5 ng/mL of purified LT-I toxin and presented 91% sensitivity, 99.5% specificity and 96.0% accuracy, thereby proving to be an excellent point-of-care test for the diagnosis of enterotoxigenic E. coli infection in low-income countries. (AU)

FAPESP's process: 13/06589-6 - Immunochromatographic assay as diagnostic tool for neglected diseases: studies of improvement, scheduling and feasibility
Grantee:Roxane Maria Fontes Piazza
Support Opportunities: Regular Research Grants