The in vitro effect of Antimicrobial Photodynamic Therapy on dental microcosm biofilms from partially erupted permanent molars: A pilot study

Author(s)

de Oliveira, Fabiana Sodre ^[1] ; Cruvinel, Thiago ^[2] ; Cusicanqui Mendez, Daniela Alejandra ^[2] ; Dionisio, Evandro Jose ^[2] ; Rios, Daniela ^[2] ; Andrade Moreira Machado, Maria Aparecida ^[2]

Affiliation

^[1] Univ Fed Uberlandia, Discipline Pediat Dent, Fac Dent, Ave Para, 1720 Bloco 2G, Sala 02, Campus Umuarama, BR-38405320 Uberlandia, MG - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Ave Dr Octavio Pinheiro Brisolla, 9-75 Vila Univ, BR-17012901 Bauru, SP - Brazil

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 21, p. 163-167, MAR 2018.
• Web of Science Citations: 5

Abstract

Background: Antimicrobial Photodynamic Therapy (aPDT) could enhance the prevention of dental caries lesions in pits and fissures of partially erupted molars, by killing microorganisms from complex dental biofilms. This pilot study aimed to evaluate the effect of Antimicrobial Photodynamic Therapy (aPDT) on the viability of specific microorganism groups of dental microcosm biofilms from occlusal surfaces of first permanent molars in eruption. Methods: Dental microcosm biofilms grown on bovine enamel blocks, from dental plaque collected on occlusal surfaces of a partially erupted lower right first permanent molar, with McBain medium plus 1% sucrose in anaerobic condition at 37 degrees C for 72 h. The experiments were performed in eight groups: L-P-= no treatment (control), L18.75P-= 18.75 J/cm(2) LED, L37.5P-= 37.5 J/cm(2) LED, L75P-= 75 J/cm(2) LED, L-P + = 200 mM TBO, L18.75P + = 200 mM TBO + 18.75 J/cm(2) LED, L37.5P + = 200 mM TBO + 37.5 J/cm(2) LED, and L75P + = 200 mM TBO + 75 J/cm(2) LED. The counts of total microorganisms, total streptococci and mutans streptococci were determined on selective media agar plates by colony-forming units per mL. The log-transformed counts were analyzed by Kruskal-Wallis and post-hoc Dunn's test (P < 0.05). Results: The counts of all microorganisms treated in the group L75P + were statistically lower than those treated in L-P-. The aPDT promoted a significant reduction of microorganisms, with a trend of dose-dependent effect. Conclusion: TBO-mediated aPDT was effective in reducing the viability of specific microbial groups in dental microcosm biofilms originated from occlusal of permanent molars in eruption. (AU)

• FAPESP's process: 14/10897-0 - Effect of antimicrobial photodynamic therapy on dentin caries microcosms
• Grantee: Thiago Cruvinel da Silva
• Support Opportunities: Regular Research Grants