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Statistical methods in evaluation of the cross-reactions between Leishmania spp. AND Ehrlichia spp. BY serological and molecular techniques

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Author(s):
Walter Bertequini Nagata
Total Authors: 1
Document type: Master's Dissertation
Press: Araçatuba. 2017-01-06.
Institution: Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária. Araçatuba
Defense date:
Advisor: Sílvia Helena Venturoli Perri; Katia Denise Saraiva Bresciani
Abstract

The aim of this study is to investigate, by statistical methods, the occurrence of cross-reactivity between Leishmania spp. and Ehrlichia spp. using serological and molecular techniques. A total of 100 blood samples from dogs were collected and processed by ELISA (indirect Enzyme-Linked Immunosorbent Assay) and PCR (polymerase chain reaction). Statistical analysis consists of McNemar tests and agreement coefficient Kappa. The statistics were considered significant when p <0.05. The sensitivity and specificity of the tests were determined by ROC curve (Receiver Operator Characteristic), considering the PCR as gold standard. From the analysis it can be seen that 48,0% of the animals were reactive in ELISA and 58,0% were positive in PCR in the case of Leishmania spp. For Ehrlichia spp., the occurrence of antibodies by ELISA was 54,0% and, by PCR, 48,0% of the dogs were positive. It can be noted also that 37,0% of the animals were positive for both parasites through molecular test and four dogs were reactive in the ELISA test for both diseases and were negative only in molecular test for Ehrlichia spp. These results in serology can suggest possible cases of cross-reactivity between Leishmania spp. and Ehrlichia spp.. However, it is more likely that these results are related to coinfection of these agents. Through the results obtained, there are more evidences of coinfection by these two pathogens in dogs than cross-reactivity between them. (AU)