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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Recognition of Candida albicans by gingival fibroblasts: The role of TLR2, TLR4/CD14, and MyD88

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Author(s):
Pinheiro, Claudia Ramos [1] ; Coelho, Ana Lucia [2] ; de Oliveira, Carine Ervolino [1] ; Gasparoto, Thais Helena [1] ; Garlet, Gustavo Pompermaier [1] ; Silva, Joao Santana [3] ; Santos, Carlos Ferreira [1] ; Cavassani, Karen Angelica [2] ; Hogaboam, Cory M. [2] ; Campanelli, Ana Paula [1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Dept Biol Sci, Bauru Sch Dent, Bauru, SP - Brazil
[2] Cedars Sinai Med Ctr, Dept Med, Adv Hlth Sci Pavil, Los Angeles, CA 90048 - USA
[3] Univ Sao Paulo, Dept Biochem & Immunol, Sch Med Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: CYTOKINE; v. 106, p. 67-75, JUN 2018.
Web of Science Citations: 5
Abstract

Recent evidence indicates that nonprofessional immune cells such as epithelial cells, endothelial cells, and fi-broblasts also contribute to innate immunity via secretion of cytokines. Fibroblasts are the principal type of cell found in the periodontal connective tissues and they are involved in the immune response during periodontal disease. The role of fibroblasts in the recognition of pathogens via Toll-like receptors (TLRs) has been established ; however, few studies have been conducted concerning the involvement of innate immune receptors in the recognition of Candida albicans by gingival fibroblast. In the current study, we investigate the functional activity of TLR2, cluster of differentiation 14 (CD14), and myeloid differentiation primary response gene 88 (MyD88) molecules in the recognition of C. albicans by gingival fibroblast. First, we identified that gingival fibroblasts expressed TLR2, TLR3, and TLR4. Our results showed that TLR agonists had no effect on these receptors' expression by TLR2, MyD88, and CD14-deficient cells. Notably, C. albicans and a synthetic triacylated lipoprotein (Pam3CSK4) induced a remarkable increase of TLR3 expression on MyD88-deficient gingival fibroblasts. TLR4 expression levels were lower than TLR2 and TLR3 levels and remained unchanged after TLR agonist stimulation. Gingival fibroblasts presented morphological similarities; however, TLR2 deficiency on these cells leads to a lower proliferative response, whereas the deficiency on CD14 expression resulted in lower levels of type I collagen by these cells. In addition, the recognition of C. albicans by gingival fibroblasts had an effect on the secretion of cytokines and it was dependent on a specific recognition molecule. Specifically, tumor necrosis factor-alpha (TNF-alpha) production after the recognition of C. albicans was dependent on MyD88, CD14, and TLR2 molecules, whereas the production of interleukin-l beta (IL-beta) and IL-13 was dependent on TLR2. These findings are the first to describe a role of gingival fibroblast in the recognition of C. albicans and the pathways involved in this process. An understanding of these pathways may lead to alternative treatments for patients with periodontal disease. (AU)