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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Endothelial modulation of a nitric oxide donor complex-induced relaxation in normotensive and spontaneously hypertensive rats

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Author(s):
Potje, Simone R. [1] ; Troiano, Jessica A. [1] ; Grando, Marcella D. [2] ; Graton, Murilo E. [1] ; da Silva, Roberto S. [2] ; Bendhack, Lusiane M. [2] ; Antoniali, Cristina [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ, UNESP, Programa Multicentr Posgrad Ciencias Fisiol SBFis, Sch Dent, Dept Basic Sci, Aracatuba - Brazil
[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Chem & Phys, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Life Sciences; v. 201, p. 130-140, MAY 15 2018.
Web of Science Citations: 0
Abstract

We hypothesized that endothelium modulates relaxation induced by a nitric oxide (NO) donor ruthenium complex (TERPY, {[}Ru(terpy)(bdq)NO](3+)) in mesenteric arteries of normotensive and spontaneously hypertensive (SHR) rats in different ways. We analyzed the mechanism involved in TERPY-induced relaxation in the second and third branches of mesenteric arteries and investigated how endothelium contributes to the TERPY vasodilator effect on SHR blood vessels. TERPY induced concentration-dependent relaxation in endothelium-denuded (E-) and endothelium-intact (E-) mesenteric arteries of normotensive rats and SHR. Pretreatment with ODQ (which inhibits soluble guanylyl cyclase) or TEA (tetraethylammonium, which blocks potassium channels) significantly reduced the TERPY vasodilator effect on E+ mesenteric arteries of normotensive rats and SHR. The presence of endothelium shifted the concentration-effect curves for TERPY in E- mesenteric arteries of normotensive rats to the right. Conversely, the presence of endothelium shifted the concentration-effect curves for TERPY in the case of SHR E- mesenteric arteries to the left, which suggested increased potency. L-NNA, a more selective endothelial NO synthase (eNOS) inhibitor, reduced TERPY potency in SHR. The presence of endothelium and notably of NOS contributed to the TERPY vasodilator action in SHR: TERPY promoted eNOS phosphorylation with consequent NO production and increased soluble guanylyl cyclase activity, which may have directly activated potassium channels. (AU)

FAPESP's process: 15/17080-2 - Effects of the stretching on the vasoconstriction modulated by the endothelium in renal hypertensive rat aorta and coronary arteries
Grantee:Lusiane Maria Bendhack
Support Opportunities: Regular Research Grants
FAPESP's process: 12/20398-6 - Participation of pathway PI3-K/AKT/NOSe, glycosylation (O-GlcNAc) and EROs in hiporeatividade vascular and hypotension observed at the end of pregnancy in rats normotensive and SHR
Grantee:Cristina Antoniali Silva
Support Opportunities: Regular Research Grants
FAPESP's process: 11/20998-0 - Analysis in vivo and in vitro effect of apocynin on endothelial dysfunction in spontaneously hypertensive rats (SHR)
Grantee:Cristina Antoniali Silva
Support Opportunities: Regular Research Grants