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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Distribution of Major Pilin Subunit Genes Among Atypical Enteropathogenic Escherichia coli and Influence of Growth Media on Expression of the ecp Operon

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Author(s):
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Munhoz, Danielle D. [1] ; Nara, Julia M. [1, 2] ; Freitas, Natalia C. [1] ; Moraes, Claudia T. P. [1] ; Nunes, Kamila O. [1] ; Yamamoto, Bruno B. [1] ; Vasconcellos, Francielli M. [1] ; Martinez-Laguna, Ygnacio [3] ; Giron, Jorge A. [3] ; Martins, Fernando H. [1] ; Abe, Cecilia M. [1] ; Elias, Waldir P. [1] ; Piazza, Roxane M. F. [1]
Total Authors: 13
Affiliation:
[1] Inst Butantan, Lab Bacteriol, Sao Paulo - Brazil
[2] Inst Biol, Lab Doencas Suinos Washington Sugay, Sao Paulo - Brazil
[3] Benemerita Univ Autonoma Puebla, Ctr Detecc Biomol, Puebla - Mexico
Total Affiliations: 3
Document type: Journal article
Source: FRONTIERS IN MICROBIOLOGY; v. 9, MAY 15 2018.
Web of Science Citations: 0
Abstract

Atypical enteropathogenic Escherichia coil (aEPEC) strains are unable to produce the bundle-forming pilus (BFP), which is responsible for the localized adherence pattern, a characteristic of the pathogenicity of typical EPEC strains. The lack of BFP in aEPEC strains suggests that other fimbrial or non-fimbrial adhesins are involved in their adhesion to the host cells. The aim of this study was to investigate the distribution of major subunit fimbrial genes known to be important adherence factors produced by several E. coil pathotypes in a collection of 72 aEPEC strains. Our results demonstrate that a high percentage (94-100%) of aEPEC strains harbored ecpA, fimA, hcpA, and lpfA fimbrial genes. Other fimbrial genes including pilS, pilV, sfpA, daaC, papA, and sfa were detected at lower frequencies (1-8%). Genes encoding fimbrial subunits, which are characteristic of enteroaggregative E. coli or enterotoxigenic E. coli were not found. No correlation was found between fimbrial gene profiles and adherence phenotypes. Since all aEPEC strains contained ecpA, the major pilin gene of the E. coil common pilus (ECP), a subset of ecpA+ strains was analyzed for transcription of ecpRABCDE and production of ECP upon growth in three different culture conditions at 37 degrees C. Transcription of ecpRABCDE occurred in all conditions; however, ECP production was medium dependent. In all, the data suggest that aEPEC strains are highly heterogeneous in terms of their fimbrial gene profiles. Despite lacking BFP production, other mechanisms of cell adherence exist in aEPEC strains to ensure host colonization, e.g., mediated by other prevalent pili such as ECP. Moreover, the production of ECP by aEPEC strains might be influenced by yet unknown post-transcriptional factors. (AU)

FAPESP's process: 13/06589-6 - Immunochromatographic assay as diagnostic tool for neglected diseases: studies of improvement, scheduling and feasibility
Grantee:Roxane Maria Fontes Piazza
Support type: Regular Research Grants
FAPESP's process: 09/14845-7 - Immunodiagnosis of diarrheagenic Escherichia coli
Grantee:Roxane Maria Fontes Piazza
Support type: Regular Research Grants
FAPESP's process: 04/12136-5 - Atypical enteropathogenetic Escherichia coli (atypical EPEC)
Grantee:Waldir Pereira Elias Junior
Support type: Research Projects - Thematic Grants