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Atypical enteropathogenetic Escherichia coli (atypical EPEC)

Grant number: 04/12136-5
Support type:Research Projects - Thematic Grants
Duration: May 01, 2005 - April 30, 2009
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Waldir Pereira Elias Junior
Grantee:Waldir Pereira Elias Junior
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Co-Principal Investigators:Antonio Fernando Pestana de Castro ; Roxane Maria Fontes Piazza
Associated grant(s):11/10592-7 - Atypical Enteropathogenic Escherichia coli that contains functional Locus of Enterocyte Effacement genes can be attaching-and-effacing negative in cultured epithelial cells, PUB.ART
07/50872-3 - Characterization of eae+eaf-stx-fecal E.coli strains isolated in Salvador, Bahia, Brazil, AR.EXT
Associated scholarship(s):07/06104-1 - Biofilm in atypical E.coli, BP.MS


The term atypical EPEC is used to define the EPEC strains that does not have the EAF plasmid. This EPEC category has been found in association with diarrhea in several countries, including Brazil. At present they are the most common bacterial agent in child endemic diarrhea in Brazil. Atypical EPEC strains may belong to serotypes of the classical EPEC. The serogroups (classical serotypes) or to serotypes in other the serogroups (non classical serotypes). The classical atypical EPEC serotypes have been identified in several studies but the non-classical ones are less known. The classical serotypes are genetically related to E. coli O157:H7 and like this serotype have been isolated from bovines and other animal species. The most common virulence factors identified so far in atypical EPEC strains are the toxins EAST and Hly but recent studies suggest that there are many others factors. The objectives of this project may be divided in two groups: one group is aimed at determining the virulence mechanisms of these organisms and includes studies on their toxins, adhesins, genetic of these virulence factors, interaction mechanisms with the host cell, cell response and proteomics. The other group of objectives includes the characterization of the serotypes and clonal groups of atypical EPEC, genetic relationships of the human and animal strains an development of animal models. More details about these objectives may be found in the text of the project. (AU)

Scientific publications (14)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MUNHOZ, DANIELLE D.; NARA, JULIA M.; FREITAS, NATALIA C.; MORAES, CLAUDIA T. P.; NUNES, KAMILA O.; YAMAMOTO, BRUNO B.; VASCONCELLOS, FRANCIELLI M.; MARTINEZ-LAGUNA, YGNACIO; GIRON, JORGE A.; MARTINS, FERNANDO H.; ABE, CECILIA M.; ELIAS, WALDIR P.; PIAZZA, ROXANE M. F. Distribution of Major Pilin Subunit Genes Among Atypical Enteropathogenic Escherichia coli and Influence of Growth Media on Expression of the ecp Operon. FRONTIERS IN MICROBIOLOGY, v. 9, MAY 15 2018. Web of Science Citations: 0.
MORAES, CLAUDIA T. P.; POLATTO, JULIANA M.; ROSSATO, SARITA S.; IZQUIERDO, MARIANA; MUNHOZ, DANIELLE D.; MARTINS, FERNANDO H.; PIMENTA, DANIEL C.; FARFAN, MAURICIO J.; ELIAS, WALDIR P.; BARBOSA, ANGELA S.; PIAZZA, ROXANE M. F. Flagellin and GroEL mediates in vitro binding of an atypical enteropathogenic Escherichia coli to cellular fibronectin. BMC Microbiology, v. 15, DEC 18 2015. Web of Science Citations: 6.
BUERIS, VANESSA; HUERTA-CANTILLO, JAZMN; NAVARRO-GARCIA, FERNANDO; RUIZ, RENATO M.; CIANCIARULLO, AURORA M.; ELIAS, WALDIR P. Late Establishment of the Attaching and Effacing Lesion Caused by Atypical Enteropathogenic Escherichia coli Depends on Protein Expression Regulated by Per. Infection and Immunity, v. 83, n. 1, p. 379-388, JAN 2015. Web of Science Citations: 8.
CULLER, HEBERT F.; MOTA, CRISTIANE M.; ABE, CECILIA M.; ELIAS, WALDIR P.; SIRCILI, MARCELO P.; FRANZOLIN, MARCIA R. Atypical Enteropathogenic Escherichia coli Strains form Biofilm on Abiotic Surfaces Regardless of Their Adherence Pattern on Cultured Epithelial Cells. BIOMED RESEARCH INTERNATIONAL, 2014. Web of Science Citations: 11.
MAGALHAES, CAROLINE A.; ROSSATO, SARITA S.; BARBOSA, ANGELA S.; DOS SANTOS, THIAGO O.; ELIAS, WALDIR P.; SIRCILI, MARCELO P.; PIAZZA, ROXANE M. F. The ability of haemolysins expressed by atypical enteropathogenic Escherichia coli to bind to extracellular matrix components. Memórias do Instituto Oswaldo Cruz, v. 106, n. 2, p. 146-152, MAR 2011. Web of Science Citations: 7.
NARA, J. M.; CIANCIARULLO, A. M.; CULLER, H. F.; BUERIS, V.; HORTON, D. S. P. Q.; MENEZES, M. A.; FRANZOLIN, M. R.; ELIAS, W. P.; PIAZZA, R. M. F. Differentiation of typical and atypical enteropathogenic Escherichia coli using colony immunoblot for detection of bundle-forming pilus expression. Journal of Applied Microbiology, v. 109, n. 1, p. 35-43, JUL 2010. Web of Science Citations: 14.
MENEZES, M. A.; ROCHA, L. B.; KOGA, P. C. M.; FERNANDES, I.; NARA, J. M.; MAGALHAES, C. A.; ABE, C. M.; AYALA, C. O.; BURGOS, Y. K.; ELIAS, W. P.; CASTRO, A. F. P.; PIAZZA, R. M. F. Identification of enteropathogenic and enterohaemorrhagic Escherichia coli strains by immunoserological detection of intimin. Journal of Applied Microbiology, v. 108, n. 3, p. 878-887, MAR 2010. Web of Science Citations: 11.
MOURA, RODRIGO A.; SIRCILI, MARCELO P.; LEOMIL, LUCIANA; MATTE, MARIA HELENA; TRABULSI, LUIZ R.; ELIAS, WALDIR P.; IRINO, KINUE; PESTANA DE CASTRO, ANTONIO F. Clonal Relationship among Atypical Enteropathogenic Escherichia coli Strains Isolated from Different Animal Species and Humans. Applied and Environmental Microbiology, v. 75, n. 23, p. 7399-7408, DEC 1 2009. Web of Science Citations: 41.
BANDO, SILVIA Y.; ANDRADE, FERNANDA B.; GUTH, BEATRIZ E. C.; ELIAS, WALDIR P.; MOREIRA-FILHO, CARLOS A.; PESTANA DE CASTRO, ANTONIO F. Atypical enteropathogenic Escherichia coli genomic background allows the acquisition of non-EPEC virulence factors. FEMS Microbiology Letters, v. 299, n. 1, p. 22-30, OCT 2009. Web of Science Citations: 18.
BARROS, SAMAR F.; ABE, CECILIA M.; ROCHA, SERGIO P. D.; RUIZ, RENATO M.; BEUTIN, LOTHAR; TRABULSI, LUIZ R.; ELIAS, WALDIR P. Escherichia coli O125ac:H6 Encompasses Atypical Enteropathogenic E-coli Strains That Display the Aggregative Adherence Pattern. Journal of Clinical Microbiology, v. 46, n. 12, p. 4052-4055, DEC 2008. Web of Science Citations: 8.
MENDES-LEDESMA, MEIRE R. B.; ROCHA, LETICIA B.; BUERIS, VANESSA; KRAUSE, GLADYS; BEUTIN, LOTHAR; FRANZOLIN, MARCIA R.; TRABULSI, LUIZ R.; ELIAS, WALDIR P.; PIAZZA, ROXANE M. F. Production and characterization of rabbit polyclonal sera against Shiga toxins Stx1 and Stx2 for detection of Shiga toxin-producing Escherichia coli. MICROBIOLOGY AND IMMUNOLOGY, v. 52, n. 10, p. 484-491, OCT 2008. Web of Science Citations: 12.
ROCHA, L. B.; PIAZZA, R. M. F. Production of Shiga toxin by Shiga toxin-expressing Escherichia coli (STEC) in broth media: from divergence to definition. Letters in Applied Microbiology, v. 45, n. 4, p. 411-417, Oct. 2007.
BANDO, SILVIA Y.; TRABULSI, LUIZ R.; MOREIRA-FILHO, CARLOS A. Genetic relationship of diarrheagenic Escherichia coli pathotypes among the enteropathogenic Escherichia coli O serogroup. Memórias do Instituto Oswaldo Cruz, v. 102, n. 2, p. 169-174, Mar. 2007.
CARVALHO, V. M.; IRINO, K.; ONUMA, D.; CASTRO, A. F. PESTANA DE. Random amplification of polymorphic DNA reveals clonal relationships among enteropathogenic Escherichia coli isolated from non-human primates and humans. Brazilian Journal of Medical and Biological Research, v. 40, n. 2, p. 237-241, Feb. 2007.

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