| Full text | |
| Author(s): Show less - |
Menezes, M. A.
[1]
;
Rocha, L. B.
[1]
;
Koga, P. C. M.
[1]
;
Fernandes, I.
[2]
;
Nara, J. M.
[1]
;
Magalhaes, C. A.
[1]
;
Abe, C. M.
[1]
;
Ayala, C. O.
[3]
;
Burgos, Y. K.
[3]
;
Elias, W. P.
[1]
;
Castro, A. F. P.
[3]
;
Piazza, R. M. F.
[1]
Total Authors: 12
|
| Affiliation: | [1] Inst Butantan, Lab Bacteriol, BR-05503900 Sao Paulo - Brazil
[2] Inst Butantan, Lab Imunopatol, BR-05503900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508 Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Journal of Applied Microbiology; v. 108, n. 3, p. 878-887, MAR 2010. |
| Web of Science Citations: | 11 |
| Abstract | |
Aims: To evaluate the sensitivity and specificity of polyclonal and monoclonal antibodies (Mabs) against intimin in the detection of enteropathogenic and enterohaemorrhagic Escherichia coli isolates using immunoblotting. Methods and Results: Polyclonal and Mabs against the intimin-conserved region were raised, and their reactivities were compared in enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC) isolates using immunoblotting analysis. In comparison with rat antiserum, rabbit anti-intimin IgG-enriched fraction had a stronger recognition pattern to a wide spectrum of intimin types in different EPEC and EHEC serotypes. On the other hand, murine monoclonal IgG2b specific to intimin, with dissociation constant of 1 center dot 3 x 10-8 mol l-1, failed in the detection of some of these isolates. Conclusion: All employed antibodies showed 100% specificity, not reacting with any of the eae-negative isolates. The sensitivity range was according to the employed antisera, and 97% for rabbit anti-intimin IgG-enriched fraction, followed by 92% and 78% sensitivity with rat antisera and Mab. Significance and Impact of the Study: The rabbit anti-intimin IgG-enriched fraction in immunoblotting analysis is a useful tool for EPEC and EHEC diagnoses. (AU) | |
| FAPESP's process: | 04/12136-5 - Atypical enteropathogenetic Escherichia coli (atypical EPEC) |
| Grantee: | Waldir Pereira Elias Junior |
| Support Opportunities: | Research Projects - Thematic Grants |