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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of enteropathogenic and enterohaemorrhagic Escherichia coli strains by immunoserological detection of intimin

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Author(s):
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Menezes, M. A. [1] ; Rocha, L. B. [1] ; Koga, P. C. M. [1] ; Fernandes, I. [2] ; Nara, J. M. [1] ; Magalhaes, C. A. [1] ; Abe, C. M. [1] ; Ayala, C. O. [3] ; Burgos, Y. K. [3] ; Elias, W. P. [1] ; Castro, A. F. P. [3] ; Piazza, R. M. F. [1]
Total Authors: 12
Affiliation:
[1] Inst Butantan, Lab Bacteriol, BR-05503900 Sao Paulo - Brazil
[2] Inst Butantan, Lab Imunopatol, BR-05503900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Applied Microbiology; v. 108, n. 3, p. 878-887, MAR 2010.
Web of Science Citations: 11
Abstract

Aims: To evaluate the sensitivity and specificity of polyclonal and monoclonal antibodies (Mabs) against intimin in the detection of enteropathogenic and enterohaemorrhagic Escherichia coli isolates using immunoblotting. Methods and Results: Polyclonal and Mabs against the intimin-conserved region were raised, and their reactivities were compared in enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC) isolates using immunoblotting analysis. In comparison with rat antiserum, rabbit anti-intimin IgG-enriched fraction had a stronger recognition pattern to a wide spectrum of intimin types in different EPEC and EHEC serotypes. On the other hand, murine monoclonal IgG2b specific to intimin, with dissociation constant of 1 center dot 3 x 10-8 mol l-1, failed in the detection of some of these isolates. Conclusion: All employed antibodies showed 100% specificity, not reacting with any of the eae-negative isolates. The sensitivity range was according to the employed antisera, and 97% for rabbit anti-intimin IgG-enriched fraction, followed by 92% and 78% sensitivity with rat antisera and Mab. Significance and Impact of the Study: The rabbit anti-intimin IgG-enriched fraction in immunoblotting analysis is a useful tool for EPEC and EHEC diagnoses. (AU)

FAPESP's process: 04/12136-5 - Atypical enteropathogenetic Escherichia coli (atypical EPEC)
Grantee:Waldir Pereira Elias Junior
Support type: Research Projects - Thematic Grants