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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Local administration of mangiferin prevents experimental inflammatory mechanical hyperalgesia through CINC-1/epinephrine/PKA pathway and TNF-alpha inhibition

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Author(s):
Rocha, Lilian Wunsch [1] ; Magayewski Bonet, Ivan Jose [1] ; Tambeli, Claudia Herrera [1] ; de-Faria, Felipe Meira [1] ; Parada, Carlos Amilcar [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Struct & Funct Biol, BR-13083862 Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: European Journal of Pharmacology; v. 830, p. 87-94, JUL 5 2018.
Web of Science Citations: 5
Abstract

Steroidal and non-steroidal anti-inflammatory drugs (NSAIDs) are widely used to control inflammatory pain, but there is a risk of gastrointestinal bleeding and increased heart failure risk. The search for new drugs remains ongoing, and natural products are a source for potential new compounds. Mangiferin, a natural xanthone Cglucoside, has demonstrated biological activity, including anti-inflammatory and analgesic properties, but ifs mechanisms are poorly understood. In this study, we investigated the mechanisms underlying the anti-inflammatory and analgesic effects of local administration of mangiferin. We employed an electronic von Frey apparatus to evaluate mechanical hyperalgesia induced by carrageenan in rats. Mangiferin (150-1200 mu g/paw), administered locally into the hindpaw, prevented hyperalgesia in a dose-dependent -150 mu g ( - 9%), 300 mu g ( - 27%, P < 0.01), 600 mu g ( - 77%, P < 0.001) and 1000 mu g ( - 93%, P < 0.001) - and local manner. Mangiferin showed decreased levels of TNF-alpha (P < 0.001) and CINC-1 (P < 0.001), but not IL-1 beta; it also prevented neutrophil migration (P < 0.01), but not the increased COX-2 expression in peripheral tissue challenged with carrageenan. To further explore the mechanisms of mangiferin actions, rats were injected with modulators of inflammation and nociception; mangiferin prevented hyperalgesia induced by IL-1 beta (P < 0.01), CINC-1 (P < 0.01), epinephrine (P < 0.01), 8-Br-cAMP (P < 0.01) or capsaicin (P < 0.01), but not that induced by PGE(2) or alpha,beta-MeATP. Our study shows that mangiferin has anti-inflammatory and analgesic properties when locally administrated. The control of the inflammatory response and mechanical hyperalgesia by mangiferin depends on the inhibition of TNF-alpha production/release and the CINC1/epinephrine/PKA pathway, supporting its marked inhibition of inflammatory mechanical hyperalgesia. (AU)

FAPESP's process: 12/04188-1 - MECHANISM OF ANALGESIC ACTION OF MANGIFERIN
Grantee:Lilian Wünsch Rocha
Support Opportunities: Scholarships in Brazil - Doctorate