Fingerprinting metabolomics in tropical mistletoes: A case study with facultative aluminum-accumulating species

Author(s)

de Souza, Marcelo Claro ^{[1, 2]} ; Rosa, Annylory Lima ^[1] ; Poschenrieder, Charlotte ^[3] ; Tolra, Roser ^[3] ; Da Costa, Fernando Batista ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, AsterBioChem Res Team, Ave Cafe S-N, BR-14040020 Ribeirao Preto, SP - Brazil

^[2] Univ Brasilia, Dept Bot, Inst Biol Sci, Campus Univ Darcy Ribeiro, BR-70910900 Brasilia, DF - Brazil

^[3] Autonomous Univ Barcelona, Bellaterra - Spain

• Document type: Journal article
• Source: PHYTOCHEMISTRY LETTERS; v. 25, p. 90-94, JUN 2018.
• Web of Science Citations: 2

Abstract

Aluminum (Al) toxicity is a hot topic due to the high sensitivity of cultivated plants. Despite the large investment in the last century in understanding the mechanisms used by sensitive and Al-excluding species to avoid Al uptake from soil, little attention has been devoted to understanding the mechanisms used by native Al-accumulating species to deal with Al-toxicity. Passovia ovata (Pohl ex DC.) Kuijt and Struthanthus polyanthus Mart. are mistletoes with a facultative Al-accumulating behavior. In the Brazilian Cerrado they are commonly found infecting Al-accumulating (Miconia albicans (Sw.) Steud.) and non-accumulating (Byrsonima verbascifolia (L.) DC.) species. Taking into account the importance of organic complexes in the detoxification of the highly toxic Al3+ ions, it is to be expected that mistletoes differ in their metabolomic profile when feeding on species differing in Al accumulation. Here we tested this hypothesis using an untargeted LC-MS approach to investigate the influence of Al on the metabolome of P. ovata and S. polyanthus infecting M. albicans and B. verbascifolia under field conditions. We observed differences in the metabolic profiles between mistletoes growing on Al-accumulating and Al-excluding hosts, and also observed a positive correlation between Al leaf-accumulation and the metabolic profile. Using the OPLS-DA, we identified quinic acid (phenolic compound) as a metabolic biomarker distinguishing mistletoes grown under high and low Al availability. (AU)

• FAPESP's process: 10/51454-3 - Morphoanatomical, metabolomic and molecular studies as subsidies to the systematic of Asteraceae species and access to their pharmacological potential
• Grantee: Beatriz Appezzato da Glória
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 14/26866-7 - Metabolomics, enzymatic targets and in silico tools in the search of bioactive compounds from plants
• Grantee: Fernando Batista da Costa
• Support type: Regular Research Grants
• FAPESP's process: 13/21293-6 - Progress on metabolomics studies of Cerrado plant species developed in different aluminium availabilities
• Grantee: Marcelo Claro de Souza
• Support type: Scholarships in Brazil - Post-Doctorate