Characterization of host cell factors involved in the budding and egress of Oropou...
| Full text | |
| Author(s): |
Teixeira Nunes, Marcio Roberto
[1, 2]
;
de Souza, William Marciel
[3]
;
Acrani, Gustavo Olszanski
[4]
;
Cardoso, Jedson Ferreira
[2]
;
da Silva, Sandro Patroca
[2]
;
Badra, Soraya Jabur
[3]
;
Moraes Figueiredo, Luiz Tadeu
[3]
;
da Costa Vasconcelos, Pedro Fernando
[5]
Total Authors: 8
|
| Affiliation: | [1] Univ Texas Med Branch, Dept Pathol, Galveston, TX 77555 - USA
[2] Minist Hlth, Evandro Chagas Inst, Ctr Technol Innovat, Ananindeua, Para - Brazil
[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Virol Res Ctr, Ribeirao Preto, SP - Brazil
[4] Univ Fed Fronteira Sul, Campus Passo Fundo, Chapeco, RS - Brazil
[5] Minist Hlth, Evandro Chagas Inst, Dept Arbovirol & Hemorrhag Fevers, Ananindeua, Para - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | PLoS One; v. 13, n. 5 MAY 24 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
Group C serogroup includes members of the Orthobunyavirus genus (family Peribunyaviridae) and comprises 15 arboviruses that can be associated with febrile illness in humans. Although previous studies described the genome characterization of Group C orthobunyavirus, there is a gap in genomic information about the other viruses in this group. Therefore, in this study, complete genomes of members of Group C serogroup were sequenced or resequenced and used for genetic characterization, as well as to understand their phylogenetic and evolutionary aspects. Thus, our study reported the genomes of three new members in Group C virus (Apeu strain BeAn848, ltaqui strain BeAn12797 and Nepuyo strain BeAn10709), as well as re-sequencing of original strains of five members: Caraparu (strain BeAn3994), Madrid (strain BT4075), Murucutu (strain BeAn974), Oriboca (strain BeAn17), and Marituba (strain BeAn15). These viruses presented a typical genomic organization related to members of the Orthobunyavirus genus. Interestingly, all viruses of this serogroup showed an open reading frame (ORF) that encodes the putative nonstructural NSs protein that precedes the nucleoprotein ORF, an unprecedented fact in Group C virus. Also, we confirmed the presence of natural reassortment events. This study expands the genomic information of Group C viruses, as well as revalidates the genomic organization of viruses that were previously reported. (AU) | |
| FAPESP's process: | 12/24150-9 - Research of virus in wild rodents, mosquitoes and ticks |
| Grantee: | William Marciel de Souza |
| Support type: | Scholarships in Brazil - Doctorate |