Structure and function of a novel antioxidant peptide from the skin of tropical frogs

Author(s):Show less -

Barbosa, Eder Alves ^{[1, 2]} ; Oliveira, Ana ^[3] ; Placido, Alexandra ^[4] ; Socodato, Renato ^{[5, 6]} ; Portugal, Camila C. ^{[5, 6]} ; Mafud, Ana Carolina ^{[7, 8]} ; Ombredane, Alicia S. ^[9] ; Moreira, Daniel C. ^[10] ; Vale, Nuno ^[11] ; Bessa, Lucinda J. ^[12] ; Joanitti, Graziella A. ^{[9, 13]} ; Alves, Claudia ^[12] ; Gomes, Paula ^[12] ; Delerue-Matos, Cristina ^[4] ; Mascarenhas, Yvonne Primerano ^[7] ; Marani, Mariela M. ^[14] ; Relvas, Joao B. ^{[5, 6]} ; Pintado, Manuela ^[3] ; Leite, Jose Roberto S. A. ^{[10, 5, 6]}

Affiliation:Show less -

^[1] EMBRAPA Recursos Genet & Biotecnol, Lab Espectrometria Massa, Brasilia, DF - Brazil

^[2] Univ Brasilia, Lab Sintese & Anal Biomol, Inst Quim, Brasilia, DF - Brazil

^[3] Univ Catolica Portuguesa, CBQF, Rua Arquiteto Lobao Vital Apartado, 2511, Oporto - Portugal

^[4] ISEP, GRAQ, LAQV REQUIMTE, Oporto - Portugal

^[5] Univ Porto, Glial Cell Biol Lab, Inst Invest & Inovacao Saude, Oporto - Portugal

^[6] Univ Porto, Inst Biol Mol & Celular, Oporto - Portugal

^[7] Univ Sao Paulo, IFSC, Sao Carlos, SP - Brazil

^[8] Swiss Trop & Publ Hlth Inst, Dept Med Parasitol & Infect Biol, CH-4051 Basel - Switzerland

^[9] Univ Brasilia, Inst Biol, Lab Nanobiotecnol, Campus Darcy Ribeiro, Brasilia, DF - Brazil

^[10] Univ Brasilia, Area Morfol, Fac Med, Brasilia, DF - Brazil

^[11] Univ Porto, Fac Farm, Dept Ciencias Medicamento, UCIBIO REQUIMTE, Lab Farmacol, Rua Jorge Viterbo Ferreira, 228, P-4050313 Oporto - Portugal

^[12] Univ Porto, Fac Ciencias, Dept Quim & Bioquim, LAQV REQUIMTE, Rua Campo Alegre, 687, P-4169007 Oporto - Portugal

^[13] Univ Brasilia, Ctr Metropolitano, Campus Ceilandia, Brasilia, DF - Brazil

^[14] Consejo Nacl Invest Cient & Tecn, IPEEC CONICET, Puerto Madryn - Argentina

• Document type: Journal article
• Source: Free Radical Biology and Medicine; v. 115, p. 68-79, FEB 1 2018.
• Web of Science Citations: 11

Abstract

The amphibian skin plays an important role protecting the organism from external harmful factors such as microorganisms or UV radiation. Based on biorational strategies, many studies have investigated the cutaneous secretion of anurans as a source of bioactive molecules. By a peptidomic approach, a novel antioxidant peptide (AOP) with in vitro free radical scavenging ability was isolated from Physalaemus nattereri. The AOP, named antioxidin-I, has a molecular weight {[}M+H](+) = 1543.69 Da and a TWYFITPYIPDK primary amino acid sequence. The gene encoding the antioxidin-I precursor was expressed in the skin tissue of three other Tropical frog species: Phyllomedusa tarsius, P. distincta and Pithecopus rohdei. cDNA sequencing revealed highly homologous regions (signal peptide and acidic region). Mature antioxidin-I has a novel primary sequence with low similarity compared with previously described amphibian's AOPs. Antioxidin-I adopts a random structure even at high concentrations of hydrophobic solvent, it has poor antimicrobial activity and poor performance in free radical scavenging assays in vitro, with the exception of the ORAC assay. However, antioxidin-I presented a low cytotoxicity and suppressed menadione-induced redox imbalance when tested with fibroblast in culture. In addition, it had the capacity to substantially attenuate the hypoxia-induced production of reactive oxygen species when tested in hypoxia exposed living microglial cells, suggesting a potential neuroprotective role for this peptide. (AU)

• FAPESP's process: 16/18023-5 - Preclinical studies with epiisopiloturine and epiisopilosine and their effect against other helminths
• Grantee: Ana Carolina Mafud
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 14/02282-6 - Structure-activity relationship studies about of Pilocarpus microphyllus (Rutaceae) alkaloid derivatives against Schistosoma mansoni
• Grantee: Ana Carolina Mafud
• Support Opportunities: Scholarships in Brazil - Post-Doctoral